Isolation and Characterization of Carboxylesterase from Vinyl Acetate-Assimilating Bacterium Isolated from Soil
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概要
- 論文の詳細を見る
A bacterium Psudomonas species which is able to assimilate vinly acetate and other esters as a sole source of carbon was isolated from soil. The bacterium contained three kinds of esterases (esterases I, II and III) which were separable on DEAE-cellulose column chromatography. Esterase II was purified and characterized.The enzyme is monomer with the molecular weight of 27,000 and it hydrolyzed various esters most efficiently at pH 8.0. The activity of the enzyme was inhibited by diisopropylflurophosphate. The purified enzyme was different from other esterases fo far found in Psedomonas sp. with respect to molecular structure and substrate specificity.
- 社団法人日本生物工学会の論文
- 1989-01-25
著者
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Inoue Y
Research Institute For Food Science Kyoto University
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Inoue Yoshiharu
Research Institute For Food S-cience Kyoto University
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MURATA KOUSAKU
Research Institute for Food Science, Kyoto University
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura Akira
Research Institute For Food Science Kyoto University
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Kimura A
Hokkaido Univ. Sapporo Jpn
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Inoue Y
Kyoto Univ. Kyoto
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Murata Kousaku
Research Institute For Food Science Kyoto Univeristy
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HATANAKA Yoshiro
Osaka Municipal Technical Research Institute
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HATANAKA YOSHIRO
Research Institute of Food Science, Kyoto University
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