Phosphorylation of AMP to ATP by Dried Escherichia coli B Cells
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概要
- 論文の詳細を見る
The glucokinase activity of Escherichia coli B cells was increased about 2.2-fold by culturing the cells in medium containing D-fructose and ammonium phosphate. After drying, these cells converted AMP to ATP, consuming glucose by the glycolytic pathway. Under the optimal conditions, AMP (30 mM) was almost completely converted into ATP within 3 h. The dried E. coli B cells harboring a hybrid plasmid (pGK100) carrying the glucokinase gene showed higher ATP-producing activity than cells without hybrid plasmid.
- 社団法人日本生物工学会の論文
- 1984-02-25
著者
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura A
Kyoto Univ. Kyoto
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Kimura Akira
Research Institute For Food Science Kyoto University
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Kimura A
Hokkaido Univ. Sapporo Jpn
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FUKUDA Yasuki
Chukyo Community College
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YAMAGUCHI Shotaro
Research and Development Division, Amano Pharmaceutical Co., Ltd.
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Shimosaka M
信州大・繊維・応生系
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Shimosaka Makoto
Research Institute For Food Science Kyoto University
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FUKUDA Yasuki
Research Institute for Food Science, Kyoto University
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Fukuda Y
Chukyo Community College
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Yamaguchi Shotaro
Research Institute For Food Science Kyoto University
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Yamaguchi Shotaro
Research And Development Division Amano Pharmaceutical Co. Ltd.
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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