Extremely Simple, Rapid, and Highly Efficient Transformation Method for the Yeast Saccharomyces cerevisiae Using Glutathione and Early Log Phase Cells
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概要
- 論文の詳細を見る
In the presence of polyethylene glycol (PEG), budding cells of Saccharomyces cerevisiae in the early log phase were transformed by exogeneous plasmid DNA without additional specific chemical or physical treatments. This capacity of the yeast cells to become competent was strictly dependent on the growth phase, being induced in the early log phase, becoming maximum between the early and mid log phases, and then disappearing rapidly in the mid log phase. The transformation was most efficient at pH 6, and the frequency increased with increasing DNA and cell concentrations. PEGs with average molecular sizes between 1000 and 3500 showed almost the same effects, and were used most efficiently at 35%. The transformation frequency of S. cerevisiae was markedly enhanced when the oxidized form of glutathione (GSSG), but not the reduced form, was included in the mixture comprising early log phase cells, plasmid DNA, and PEG, and the transformation system with GSSG could be used as a convenient transformation method for the yeast S. cerevisiae.
- 社団法人日本生物工学会の論文
- 2002-08-25
著者
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橋本 渉
京大院・農・食生科
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Murata K
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Murata Kousaku
Div. Of Food Sci. And Biotech. Grad. Sch. Of Agriculture Kyoto Univ.
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HASHIMOTO Wataru
Department of Cardiovascular Surgery, Graduate School of Medicine, Nagasaki University
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FUKUDA Yasuki
Chukyo Community College
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KAWAI SHIGEYUKI
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Gr
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MURATA KOUSAKU
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Gr
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HAYAMA YOSHIYUKI
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto Unive
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Kawai Shigeyuki
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Hashimoto Wataru
Department Of Cardiovascular Surgery Graduate School Of Medicine Nagasaki University
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Matsushita Kazunobu
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsushita K
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Fukuda Y
Chukyo Community College
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Kawai Shigeyuki
Laboratory Of Basic And Applied Molecular Biotechnology Graduate School Of Agriculture Kyoto Univers
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Kawai Shigeyuki
Department Of Basic And Applied Molecular Biotechnology Division Of Food And Biological Science Grad
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Murata Kousaku
Department Of Basic And Applied Molecular Biotechnology Division Of Food And Biological Science Grad
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松下 一信
山口大 農
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Hayama Yoshiyuki
Department Of Basic And Applied Molecular Biotechnology Graduate School Of Agriculture Kyoto Univers
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