Primary Structure of Inorganic Polyphosphate/ATP-NAD Kinase from Micrococcus flavus, and Occurrence of Substrate Inorganic Polyphosphate for the Enzyme(Biochemistry & Molecular Biology)
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概要
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The gene encoding an inorganic polyphosphate/ATP-NAD kinase was cloned from Micrococcus flavus, and its primary structure was analyzed. Alignment of the primary structure with those of other characterized NAD kinases revealed candidate amino acid residues, mainly charged ones, that would be related to inorganic polyphosphate use. The alignment also showed that the primary structure found carried a protruding C-terminal polypeptide. Although the C-terminal polypeptide was demonstrated to be dispensable for the kinase activities, and was proposed to be removed in M. flavus, the entire primary structure including the C-terminal polypeptide was homologous with that of the ATP synthase β chain. The inorganic polyphosphate used by the inorganic polyphosphate/ATP-NAD kinase as a phosphoryl donor was isolated from cells of M. flavus, suggesting that the ability of the enzyme to use inorganic polyphosphate is of physiological significance and is not an evolutionary trait alone.
- 社団法人日本農芸化学会の論文
- 2003-08-23
著者
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Murata K
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Murata Kousaku
Div. Of Food Sci. And Biotech. Grad. Sch. Of Agriculture Kyoto Univ.
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MORI SHIGETAROU
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Gr
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KAWAI SHIGEYUKI
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Gr
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MURATA KOUSAKU
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Gr
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Kawai Shigeyuki
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Matsushita Kazunobu
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Murata Kousaku
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Momma Keiko
Laboratory Of Basic And Applied Molecular Biotechnology Graduate School Of Agriculture Kyoto Univers
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Mori Shigetarou
Department Of Basic And Applied Molecular Biotechnology Division Of Food And Biological Science Grad
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Kawai Shigeyuki
Laboratory Of Basic And Applied Molecular Biotechnology Graduate School Of Agriculture Kyoto Univers
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Kawai Shigeyuki
Department Of Basic And Applied Molecular Biotechnology Division Of Food And Biological Science Grad
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Murata Kousaku
Department Of Basic And Applied Molecular Biotechnology Division Of Food And Biological Science Grad
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松下 一信
山口大 農
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MORI Shigetarou
Department of Bacteriology II, National Institute of Infectious Diseases
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