Purification and Characterization of Two NAD-Dependent Alcohol Dehydrogenases (ADHs) Induced in the Quinoprotein ADH-Deficient Mutant of Acetobacter pasteurianus SKU1108(Microbiology & Fermentation Technology)

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概要

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• High NAD-dependent alcohol dehydrogenase (ADH) activity was found in the cytoplasm when a membrane bound, quinoprotein, ADH-deficient mutant strain of Acetobacter pasteurianus SKU1108 was grown on ethanol. Two NAD-dependent ADHs were separated and purified from the supernatant fraction of the cells. One (ADH I) is a trimer, consisting of an identical subunit of 42 kDa, while the other (ADH II) is a homodimer, having a subunit of 31 kDa. One of the two ADHs, ADH II, easily lost the activity during the column chromatographies, which could be stabilized by the addition of DTT and MgCl_2 in the column buffer. ADH I but not ADH II contained approximately one zinc atom per subunit. The N-terminal amino acid analysis indicated that ADH I and ADH II have homology to the long-chain and short-chain ADH families, respectively. ADH I showed a preference for primary alcohols, while ADH II had a preference for secondary alcohols. The two ADHs showed clear difference in their kinetics on ethanol, acetaldehyde, NAD, and NADH. The physiological function of both ADH I and ADH II are also discussed.

• 社団法人日本農芸化学会の論文
• 2003-05-23

著者

• Theeragool G.

  カセサート大学理

• TOYAMA Hirohide

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• THEERAGOOL Gunjana

  Department of Microbiology, Faculty of Science, Kasetsart University

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Toyama Hirohide

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Department Of Agricultural Chemistry Yamaguchi University

• LIMTONG SAVITREE

  Department of Microbiology, Faculty of Science, Kasetsart University

• Theeragool G.

  カセサート大理微生物

• Theeragool Gunjana

  カセサート大理 微生物

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita K

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• CHINNAWIROTPISAN PIYAWAN

  Department of Microbiology, Faculty of Science, Kasetsart University

• Theeragool Gunjana

  Department Of Microbiology Faculty Of Science Kasetsart University

• 松下 一信

  山口大 農

• Limtong S

  Department Of Microbiology Faculty Of Science Kasetsart University

• Limtong Savitree

  Department Of Microbiology Faculty Of Science Kasetsart University

• Chinnawirotpisan Piyawan

  Department Of Microbiology Faculty Of Science Kasetsart University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

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