Molecular Target of Piperine in the Inhibition of Lipid Droplet Accumulation in Macrophages(Biochemistry)
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概要
- 論文の詳細を見る
An alkaloid piperine isolated from the Piper Nigrum was found to inhibit lipid droplet accumulation in mouse macrophages, and especially inhibited cholesteryl ester (CE) synthesis (IC_<50>: 25 μM). The metabolism of cholesterol from lysosome to lipid droplet was inhibited with a similar IC_<50> (18 μM), indicating that the site of inhibition is one of the steps between the lysosomes and the endoplasmic reticulum. Therefore, effects of piperine on acyl-CoA: cholesterol acyltransferase (ACAT) activity in the microsomes prepared from mouse macrophage and liver were studied, to show that the compounds inhibited the activity in both cases (IC_<50>: 9.1, 7.0 μM, respectively). Furthermore, piperine was found to inhibit both ACAT1 and ACAT2 isozymes to a similar extent (IC_<50>: 16, 18 μM, respectively) in cell-based assays using ACAT1- or ACAT2-expressing cells. Thus, it was suggested that piperine inhibited macrophage ACAT to decrease CE synthesis, leading to a reduction of lipid droplets.
- 2008-06-01
著者
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外山 博英
山口大学農学部生物機能科学科
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MATSUDA Daisuke
Graduate School of Pharmacy, Kitasato University
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OHTE Satoshi
Graduate School of Pharmacy, Kitasato University
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OHSHIRO Taichi
Graduate School of Pharmacy, Kitasato University
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JIANG Wei
National Key Laboratory for Screening of New Microbial Drugs, Institute of Medical Biotechnology, Ch
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RUDEL Lawrence
Department of Pathology, Arteriosclerosis Research Program, Wake Forest University School of Medicin
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HONG Bin
National Key Laboratory for Screening of New Microbial Drugs, Institute of Medical Biotechnology, Ch
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SI Shuyi
National Key Laboratory for Screening of New Microbial Drugs, Institute of Medical Biotechnology, Ch
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TOMODA Hiroshi
Graduate School of Pharmacy, Kitasato University
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Toyama Hirohide
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Takayanagi H
Research Center For Biological Function The Kitasato Institute
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Takayanagi H
Kitasato Univ. Tokyo Jpn
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Rudel Lawrence
Atherosclerosis Research Program Wake Forest University School Of Medicine
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Hirose T
School Of Pharmacy Kitasato University
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Tanaka Y
School Of Pharmaceutical Sciences Kitasato University The Kitasato Institute
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Omura S
Kitasato Institute For Life Sciences Kitasato University
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Ohshiro Taichi
Graduate School Of Pharmaceutical Sciences Kitasato University:(present Office)department Of Medhici
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Hong Bin
National Key Laboratory For Screening Of New Microbial Drugs Institute Of Medical Biotechnology Chin
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Si Shuyi
National Key Laboratory For Screening Of New Microbial Drugs Institute Of Medical Biotechnology Chin
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Matsuda Daisuke
Graduate School Of Pharmaceutical Sciences Kitasato University
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Jiang Wei
National Key Laboratory For Screening Of New Microbial Drugs Institute Of Medical Biotechnology Chin
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Tomoda Hiroshi
Graduate School Of Pharmaceutical Sciences Kitasato University
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Ohshiro Taichi
Graduate School Of Pharmaceutical Sciences Kitasato University
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Tomoda Hiroshi
Graduate School Of Pharmaceutical Sciences Kitasato Univ.
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OHTE Satoshi
Graduate School of Pharmaceutical Sciences, Kitasato University
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Tomoda Hiroshi
Graduate School of Pharmaceutical Science, Kitasato University
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