Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway

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概要

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• A method for enzymatic preparation of 3-dehydroquinate and 3-dehydroshikimate in the shikimate pathway was established by controlling the enzyme activity of 3-dehydroquinate dehydratase. When quinate was incubated with the membrane fraction of acetic acid bacteria at pH 5.0, 3-dehydroquinate was formed as the predominant product. 3-Dehydroshikimate was the sole product when incubated at pH 8.0. Mutual separation of the metabolic intermediates was also exemplified.

• 社団法人 日本農芸化学会の論文
• 2006-12-23

著者

• TOYAMA Hirohide

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agricultre, Yamaguchi University

• Toyama Hirohide

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Department Of Agricultural Chemistry Yamaguchi University

• Hirose T

  School Of Pharmacy Kitasato University

• Tanaka Y

  School Of Pharmaceutical Sciences Kitasato University The Kitasato Institute

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Tanaka H

  External Science Affairs Fujisawa Pharmaceutical Co. Ltd.

• Murata Kousaku

  Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O

• Momma Keiko

  Laboratory Of Basic And Applied Molecular Biotechnology Graduate School Of Agriculture Kyoto Univers

• Matsushita K

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• 松下 一信

  山口大 農

• Adachi O

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Ano Yoshitaka

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Applied Bioscience, Faculty of Agriculture, Ehime University

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