Solubilization, Purification, and Properties of Membrane-Bound D-Glucono-δ-lactone Hydrolase from Gluconobacter oxydans

スポンサーリンク

概要

• 論文の詳細を見る

• Membrane-bound glucono-δ-lactonase (MGL) was purified to homogeneity from the membrane fraction of Gluconobacter oxydans IFO 3244. After solubilization with 1 M CaCl2, MGL was purified in the presence of Ca2+ and detergent. A single band corresponding to 60 kDa appeared in SDS–PAGE. The molecular weight of MGL was judged to be 120k. Differently from cytoplasmic lactonases, MGL showed optimum pH in an acidic range of 5–5.5. It was highly sensitive to metal-chelating agents such as EDTA, and the lost MGL activity was restored to the original level by the addition of divalent cations such as Ca2+ or Mg2+. The purified MGL was strictly dependent on Ca2+ and underwent rapid denaturing precipitation on Ca2+ depletion even in the presence of detergent. This communication can be the first one dealing with the solubilization, purification and properties of MGL.

• 社団法人 日本農芸化学会の論文

著者

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agricultre, Yamaguchi University

• SHINAGAWA Emiko

  Department of Chemical and biological Engineering, Ube National College of Technology

• Yakushi Toshiharu

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University

• Yakushi Toshiharu

  Dep. Of Biological Chemistry Fac. Of Agriculture Yamaguchi Univ.

関連論文

• L-Erythrulose Production by Oxidative Fermentation is Catalyzed by PQQ-Containing Membrane-bound Dehydrogenase(Microbiology & Fermentation Technology)
• Purification and Characterization of Membrane-bound Quinoprotein Cyclic Alcohol Dehydrogenase from Gluconobacter frateurii CHM 9(Microbiology & Fermentation Technology)
• Isolation and Characterization of Thermotolerant Gluconobacter Strains Catalyzing Oxidative Fermentation at Higher Temperatures
• Distinct Physiological Roles of Two Membrane-Bound Dehydrogenases Responsible for D-Sorbitol Oxidation in Gluconobacter frateurii
• Disruption of the Membrane-Bound Alcohol Dehydrogenase-Encoding Gene Improved Glycerol Use and Dihydroxyacetone Productivity in Gluconobacter oxydans
• Molecular Properties of Membrane-Bound FAD-Containing D-Sorbitol Dehydrogenase from Thermotolerant Gluconobacter frateurii Isolated from Thailand
• 3-Dehydroquinate Production by Oxidative Fermentation and Further Conversion of 3-Dehydroquinate to the Intermediates in the Shikimate Pathway(Microbiology & Fermentation Technology)
• Purification and Characterization of Membrane-bound Quinoprotein Quinate Dehydrogenase(Biochemistry & Molecular Biology)
• Molecular Cloning of Quinohemoprotein Alcohol Dehydrogenase, ADH IIB, from Pseudomonas putida HK5(Biochemistry & Molecular Biology)
• Alterations of cellular physiology in Escherichia coli in response to oxidative phosphorylation impaired by defective F1-ATPase
• Methionaquinone is a Direct Natural Electron Acceptor for the Membrane-bound Hydrogenase in Hydrogenobacter thermophilus Strain TK-6(Biological Chemistry)
• 2P185 The role of charged residues in C-terminus of PomA in V. alginolyticus(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Production of 4-Keto-D-arabonate by Oxidative Fermentation with Newly Isolated Gluconacetobacter liquefaciens
• Biochemical and Spectroscopic Properties of Cyanide-Insensitive Quinol Oxidase from Gluconobacter oxydans
• A Tightly Bound Quinone Functions in the Ubiquinone Reaction Sites of Quinoprotein Alcohol Dehydrogenase of an Acetic Acid Bacterium, Gluconobacter suboxydans
• 2P030 酢酸菌Gluconobacter frateurii由来L-sorbose reductaseのX線結晶構造解析(蛋白質-構造機能相関,第48回日本生物物理学会年会)
• Preparation and Preservation of Freeze-dried Cells of Acetic Acid Bacteria with Aldehyde Oxidase Activity
• A Novel Polysaccharide Involved in the Pellicle Formation of Acetobacter aceti
• Vanilin Formation by Microbial Amine Oxidases from Vanillylamine
• Siccanin Rediscovered as a Species-Selective Succinate Dehydrogenase Inhibitor
• Use of a Gluconobacter frateurii Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol
• Characterization of Membrane-bound Spermidine Dehydrogenase of Citrobacter freundii
• Solubilization, Purification, and Properties of Membrane-Bound D-Glucono-δ-lactone Hydrolase from Gluconobacter oxydans
• Quinoprotein Alcohol Dehydrogenase of Acetic Acid Bacteria: Kinetic Study on the Enzyme Purified from Acetobacter methanolicus
• Enzymes Responsible for Acetate Oxidation by Acetic Acid Bacteria
• Microbiological Aspects of Acetate Oxidation by Acetic Acid Bacteria, Unfavorable Phenomena in Vinegar Fermentation
• Purification and Characterization of Methylamine Oxidase Induced in Aspergillus niger AKU 3302
• NADPH Oxidase System as a Superoxide-generating Cyanide-Resistant Pathway in the Respiratory Chain of Corynebacterium glutamicum
• Role of Cytochrome c-553(CO), the Second Subunit of Alcohol Dehydrogenase, in the Azide-Insensitive Respiratory Chain and in Oxidative Fermentation of Gluconobacter Species
• Ethanol Oxidase Respiratory Chain of Acetic Acid Bacteria. Reactivity with Ubiquinone of Pyrroloquinoline Quinone-dependent Alcohol Dehydrogenases Purified from Acetobacter aceti and Gluconobacter suboxydans
• Formaldehyde Elimination with Formaldehyde and Formate Oxidase in Membrane of Acetic Acid Bacteria(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Energy Metabolism of a Unique Acetic Acid Bacterium, Asaia bogorensis, That Lacks Ethanol Oxidation Activity
• A Novel 3-Dehydroquinate Dehydratase Catalyzing Extracellular Formation of 3-Dehydroshikimate by Oxidative Fermentation of Gluconobacter oxydans IFO 3244
• Involvement of Multiple Copper/Topa Quinone-Contanining and Flavin-Containing Amine Oxidases and NAD(P)^+ Aldehyde Dehydrogenases in Amine Degradation by Filamentous Fungi
• Azurin Involved in Alcohol Oxidation System in Pseudomonas putida HK5 : Expression Analysis and Gene Cloning(Biochemistry & Molecular Biology)
• Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway
• Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production
• High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria
• Conversion of Capsular Polysaccharide, Involved in Pellicle Formation, to Extracellular Polysaccharide by galE Deletion in Acetobacter tropicalis
• Growth Promoting Substance in Yeast Extract for Methylotrophic Growth of Candida boidinii
• A Novel Type of Formaldehyde-Oxidizing Enzyme from the Membrane of Acetobacter sp. SKU 14
• Electron Transfer Ability from NADH to Menaquinone and from NADPH to Oxygen of Type II NADH Dehydrogenase of Corynebacterium glutamicum
• Acetic Acid Fermentation of Acetobacter pasteurianus: Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation
• Quinoprotein Alcohol Dehydrogenase Is Involved in Catabolic Acetate Production, while NAD-Dependent Alcohol Dehydrogenase in Ethanol Assimilation in Acetobacter pasteurianus SKU1108(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Purification and Characterization of Two NAD-Dependent Alcohol Dehydrogenases (ADHs) Induced in the Quinoprotein ADH-Deficient Mutant of Acetobacter pasteurianus SKU1108(Microbiology & Fermentation Technology)
• Purification and Characterization of a Novel Polysaccharide Involved in the Pellicle Produced by a Thermotolerant Acetobacter Strain(Microbiology & Fermentation Technology)
• Purification and Characterization of Membrane-bound Malate Dehydrogenase from Acetobacter sp. SKU 14(Microbiology & Fermentation Technology)
• Membrane-bound Sugar Alcohol Dehydrogenase in Acetic Acid Bacteria catalyzes L-Ribulose Formation and NAD-Dependent Ribitol Dehydrogenase is Independent of the Oxidative Fermentation
• Crystallization and Properties of NADPH-Dependent L-Sorbose Reductase from Gluconobacter melanogenus IFO 3294
• Crystallization and Properties of NAD-Dependent D-Sorbitol Dehydrogenase from Gluconobacter suboxydans IFO 3257
• Development of Thermotolerant Acetic Acid Bacteria Useful for Vinegar Fermentation at Higher Temperatures
• Further Properties of Spermidine Dehydrogenase from Citrobacter freundii IFO 12681
• A Tightly Bound Quinone Functions in the Ubiquinone Reaction Sites of Quinoprotein Alcohol Dehydrogenase of an Acetic Acid Bacterium, Gluconobacter suboxydans
• A Novel 3-Dehydroquinate Dehydratase Catalyzing Extracellular Formation of 3-Dehydroshikimate by Oxidative Fermentation of Gluconobacter oxydans IFO 3244
• Energy Metabolism of a Unique Acetic Acid Bacterium, Asaia bogorensis, That Lacks Ethanol Oxidation Activity
• Distinct Physiological Roles of Two Membrane-Bound Dehydrogenases Responsible for D-Sorbitol Oxidation in Gluconobacter frateurii
• Use of a Gluconobacter frateurii Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol
• Prosthetic Group of Aldehyde Dehydrogenase in Acetic Acid Bacteria Not Pyrroloquinoline Quinone
• Purification and Characterization of Membrane-Bound 3-Dehydroshikimate Dehydratase from Gluconobacter oxydans IFO 3244, A New Enzyme Catalyzing Extracellular Protocatechuate Formation
• Production of 4-Keto-D-arabonate by Oxidative Fermentation with Newly Isolated Gluconacetobacter liquefaciens
• Conversion of Quinate to 3-Dehydroshikimate by Ca-Alginate-Immobilized Membrane of Gluconobacter oxydans IFO 3244 and Subsequent Asymmetric Reduction of 3-Dehydroshikimate to Shikimate by Immobilized Cytoplasmic NADP-Shikimate Dehydrogenase
• Acetic Acid Fermentation of Acetobacter pasteurianus : Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation
• Solubilization, Purification, and Properties of Membrane-Bound D-Glucono-δ-lactone Hydrolase from Gluconobacter oxydans
• Preparation of Enzymes Required for Enzymatic Quantification of 5-Keto-D-gluconate and 2-Keto-D-gluconate
• Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
• Purification and Properties of Two Different Dihydroxyacetone Reductases in Gluconobacter suboxydans Grown on Glycerol
• The Occurrence of a Novel NADH Dehydrogenase, Distinct from the Old Yellow Enzyme, in Gluconobacter Strains
• Purification and Characterization of Laccase from White Rot Fungus Trametes sanguinea M85-2
• Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
• Purification and Characterization of Fe(III)-EDTA Reductase from Bacillus sp. B-3
• Global Analysis of the Genes Involved in the Thermotolerance Mechanism of Thermotolerant Acetobacter tropicalis SKU1100
• Formation of 4-Keto-D-aldopentoses and 4-Pentulosonates (4-Keto-D-pentonates) with Unidentified Membrane-Bound Enzymes from Acetic Acid Bacteria
• Mechanism of increased respiration in an H^+-ATPase-defective mutant of Corynebacterium glutamicum(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Enzymatic Synthesis of 4-Pentulosonate (4-Keto-D-pentonate) from D-Aldopentose and D-Pentonate by Two Different Pathways Using Membrane Enzymes of Acetic Acid Bacteria
• Pentose Oxidation by Acetic Acid Bacteria Led to a Finding of Membrane-Bound Purine Nucleosidase

もっと見る 閉じる

スポンサーリンク