Conversion of Quinate to 3-Dehydroshikimate by Ca-Alginate-Immobilized Membrane of Gluconobacter oxydans IFO 3244 and Subsequent Asymmetric Reduction of 3-Dehydroshikimate to Shikimate by Immobilized Cytoplasmic NADP-Shikimate Dehydrogenase

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概要

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• The membrane fraction of Gluconobacter oxydans IFO 3244, involving membrane-bound quinoprotein quinate dehydrogenase and 3-dehydroquinate dehydratase, was immobilized into Ca-alginate beads. The Ca-alginate-immobilized bacterial membrane catalyzed a sequential reaction of quinate oxidation to 3-dehydroquinate and its spontaneous conversion to 3-dehydroshikimate under neutral pH. An almost 100% conversion rate from quinate to 3-dehydroshikimate was observed. NADP-Dependent cytoplasmic enzymes from the same organism, shikimate dehydrogenase and D-glucose dehydrogenase, were immobilized together with different carriers as an asymmetric reduction system forming shikimate from 3-dehydroshikimate. Blue Dextran 2000, Blue Dextran-Sepharose-4B, DEAE-Sephadex A-50, DEAE-cellulose, and hydroxyapatite were effective carriers of the two cytoplasmic enzymes, and the 3-dehydroshikimate initially added was converted to shikimate at 100% yield. The two cytoplasmic enzymes showed strong affinity to Blue Dextran 2000 and formed a soluble form of immobilized catalyst having the same catalytic efficiency as that of the free enzymes. This paper may be the first one on successful immobilization of NAD(P)-dependent dehydrogenases.

• 社団法人 日本農芸化学会の論文
• 2010-12-23

著者

• 松下 一信

  山口大学農学部生物機能科学科応用微生物学研究室

• 品川 恵美子

  宇部高専物質工学科

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agricultre, Yamaguchi University

• SHINAGAWA Emiko

  Department of Chemical and biological Engineering, Ube National College of Technology

• Yakushi Toshiharu

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University

• Yakushi Toshiharu

  Dep. Of Biological Chemistry Fac. Of Agriculture Yamaguchi Univ.

• Murata K

  Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O

• Murata K

  Laboratory Of Basic And Applied Molecular Biotechnology Division Of Food Science And Biotechnology G

• Murata Kousaku

  Div. Of Food Sci. And Biotech. Grad. Sch. Of Agriculture Kyoto Univ.

• Adachi Osao

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Department Of Agricultural Chemistry Yamaguchi University

• Shinagawa Emiko

  Department Of Chemical And Biological Engineering Ube National College Of Technology

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Murata Kousaku

  Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O

• Matsushita K

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Takaki Yoshihiro

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Yakushi Toshiharu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• 松下 一信

  山口大 農

• Adachi O

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Ano Yoshitaka

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Shinagawa Emiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Applied Bioscience, Faculty of Agriculture, Ehime University

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