Conversion of Quinate to 3-Dehydroshikimate by Ca-Alginate-Immobilized Membrane of Gluconobacter oxydans IFO 3244 and Subsequent Asymmetric Reduction of 3-Dehydroshikimate to Shikimate by Immobilized Cytoplasmic NADP-Shikimate Dehydrogenase
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概要
- 論文の詳細を見る
The membrane fraction of Gluconobacter oxydans IFO 3244, involving membrane-bound quinoprotein quinate dehydrogenase and 3-dehydroquinate dehydratase, was immobilized into Ca-alginate beads. The Ca-alginate-immobilized bacterial membrane catalyzed a sequential reaction of quinate oxidation to 3-dehydroquinate and its spontaneous conversion to 3-dehydroshikimate under neutral pH. An almost 100% conversion rate from quinate to 3-dehydroshikimate was observed. NADP-Dependent cytoplasmic enzymes from the same organism, shikimate dehydrogenase and D-glucose dehydrogenase, were immobilized together with different carriers as an asymmetric reduction system forming shikimate from 3-dehydroshikimate. Blue Dextran 2000, Blue Dextran-Sepharose-4B, DEAE-Sephadex A-50, DEAE-cellulose, and hydroxyapatite were effective carriers of the two cytoplasmic enzymes, and the 3-dehydroshikimate initially added was converted to shikimate at 100% yield. The two cytoplasmic enzymes showed strong affinity to Blue Dextran 2000 and formed a soluble form of immobilized catalyst having the same catalytic efficiency as that of the free enzymes. This paper may be the first one on successful immobilization of NAD(P)-dependent dehydrogenases.
- 社団法人 日本農芸化学会の論文
- 2010-12-23
著者
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松下 一信
山口大学農学部生物機能科学科応用微生物学研究室
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品川 恵美子
宇部高専物質工学科
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MATSUSHITA Kazunobu
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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ADACHI Osao
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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ANO Yoshitaka
Department of Biological Chemistry, Faculty of Agricultre, Yamaguchi University
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SHINAGAWA Emiko
Department of Chemical and biological Engineering, Ube National College of Technology
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Yakushi Toshiharu
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University
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Yakushi Toshiharu
Dep. Of Biological Chemistry Fac. Of Agriculture Yamaguchi Univ.
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Murata K
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Murata K
Laboratory Of Basic And Applied Molecular Biotechnology Division Of Food Science And Biotechnology G
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Murata Kousaku
Div. Of Food Sci. And Biotech. Grad. Sch. Of Agriculture Kyoto Univ.
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Adachi Osao
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Adachi Osao
Department Of Agricultural Chemistry Yamaguchi University
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Shinagawa Emiko
Department Of Chemical And Biological Engineering Ube National College Of Technology
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Matsushita Kazunobu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsushita Kazunobu
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Murata Kousaku
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Matsushita K
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Takaki Yoshihiro
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Yakushi Toshiharu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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松下 一信
山口大 農
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Adachi O
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Ano Yoshitaka
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Shinagawa Emiko
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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Matsushita Kazunobu
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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ANO Yoshitaka
Department of Applied Bioscience, Faculty of Agriculture, Ehime University
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