Quinoprotein Alcohol Dehydrogenase Is Involved in Catabolic Acetate Production, while NAD-Dependent Alcohol Dehydrogenase in Ethanol Assimilation in Acetobacter pasteurianus SKU1108(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• The relationship between quinoprotein alcohol dehydrogenase (ADH) and NAD-dependent ADH was studied by constructing quinoprotein ADH-deficient mutants. Quinoprotein ADH-deficient mutants were successfully constructed from Acetobacterpasteurianus SKU1108 by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis and also by adhA gene disruption with a kana mycin cassette. The NTG mutant exhibited a complete loss of its acetate-producing ability and acetic acid resistance, while the disruptant also exhibited a loss of its acetic acid resistance but re tained a weak ADH activity. The immunoblot analysis of quinoprotein ADH indicated that there are no appreciable ADH subunits in the membranes of both mutant strains. The NTG mutant grew better than the wild-type strain in ethanol-containing medium, despite the absence of quinoprotein ADH. In the mutant, the activities of two NAD-dependent ADHs, present in a small amount in the wild-type strain, markedly increased in the cytoplasm when cultured in a medium containing ethanol, concomitant to the increase in the activities of the key enzymes in TCA and glyoxylate cycles. The disruptant showed a poorer growth than the wild-type strain, producing a lower amount of acetic acid in ethanol culture, and it induced one of the two NAD-dependent ADHs and some of the acetate-assimilating enzymes induced in the NTG mutant. This study clearly showed that quinoprotein ADH is extensively involved in acetic acid production, while NAD-dependent ADH only in ethanol assimilation through the TCA and glyoxylate cycles in acetic acid bacteria. The differences between the NTG mutant and the disruptant are also discussed.

• 社団法人日本生物工学会の論文
• 2003-12-25

著者

• Theeragool G.

  カセサート大学理

• TOYAMA Hirohide

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• THEERAGOOL Gunjana

  Department of Microbiology, Faculty of Science, Kasetsart University

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Toyama Hirohide

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Department Of Agricultural Chemistry Yamaguchi University

• LIMTONG SAVITREE

  Department of Microbiology, Faculty of Science, Kasetsart University

• Theeragool G.

  カセサート大理微生物

• Theeragool Gunjana

  カセサート大理 微生物

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita K

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• CHINNAWIROTPISAN PIYAWAN

  Department of Microbiology, Faculty of Science, Kasetsart University

• Theeragool Gunjana

  Department Of Microbiology Faculty Of Science Kasetsart University

• 松下 一信

  山口大 農

• Limtong S

  Department Of Microbiology Faculty Of Science Kasetsart University

• Limtong Savitree

  Department Of Microbiology Faculty Of Science Kasetsart University

• Chinnawirotpisan Piyawan

  Department Of Microbiology Faculty Of Science Kasetsart University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

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