Soluble and Membrane-bound Quinoprotein D-Glucose Dehydrogenases of the Acinetobacter calcoaceticus : The Binding Process of PQQ to the Apoenzymes
スポンサーリンク
概要
- 論文の詳細を見る
Acinetobacter calcoaceticus LMD 79.41 is a unique bacterium containing a soluble quinoprotein D-glucose dehydrogenase (sGDH) in addition to the membrane-bound quinoprotein D-glucose dehydrogenase (mGDH) which is distributed extensively in Gram-negative bacteria. sGDH has been shown to be a distinct enzyme from mGDH, though both enzymes contain a tightly bound pyrroloquinoline quinone (PQQ) as their prosthetic group. In this study, sGDH was detectable in all strains tested of A. calcoaceticus but not in other Gram-negative bacteria tested, indicating that sGDH can be useful as a taxonomic marker for A. calcoaceticus. The binding process of PQQ with both enzymes was examined by using the apoenzymes purified from a PQQ-deficient mutant strain of A. calcoaceticus. sGDH was able to bind two moles of PQQ in one mole of the homodimer with a fairly high affinity. The binding reaction was much faster at alkaline pH than at acidic pH, and required the presence of some divalent cations such as Cd^<2+>, Ca^<2+>, Sr^<2+>, or Mn^<2+>. On the other hand, mGDH bound one mol of PQQ in the monomeric enzyme with a relatively slow reacting process, which was optimum at acidic pH and in the presence of different types of divalent cations such as Mg^<2+>, Ca^<2+>, Zn^<2+>, or Sr^<2+> . Thus, it is suggested that sGDH and mGDH have distinct structures around their PQQ binding site. Furthermore, binding of PQQ affects the conformation of both enzymes, which can be shown from the diminishing intrinsic fluorescence of the enzymes and the increase in resistance against proteolysis upon PQQ binding. Data also suggest that the conformational changes caused by PQQ-binding are more dramatic in sGDH than in mGDH. Based on the results obtained, the differences in PQQ-binding mode between the enzymes and the physiological meanings of sGDH are discussed.
- 社団法人日本農芸化学会の論文
- 1995-08-23
著者
-
Toyama Hirohide
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
-
Adachi Osao
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
-
Adachi Osao
Department Of Agricultural Chemistry Yamaguchi University
-
Ameyama M
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
-
Matsushita Kazunobu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
-
Ameyama Minoru
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
-
Dewanti Aster
Department of Microbiology and Enzymology, Delft University of Technology
-
Duine A.
Department of Microbiology and Enzymology, Delft University of Technology
-
Duine A.
Department Of Microbiology And Enzymology Delft University Of Technology
-
Dewanti Aster
Department Of Microbiology And Enzymology Delft University Of Technology
-
Matsushita Kazunobu
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
-
Ameyama Minoru
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
関連論文
- 10.酢酸菌のグルコン酸酸化にかかわる第3の膜酵素・FADグルコン酸脱水素酵素(第419回研究協議会研究発表要旨,ビタミンB研究委員会)
- L-Erythrulose Production by Oxidative Fermentation is Catalyzed by PQQ-Containing Membrane-bound Dehydrogenase(Microbiology & Fermentation Technology)
- Purification and Characterization of Membrane-bound Quinoprotein Cyclic Alcohol Dehydrogenase from Gluconobacter frateurii CHM 9(Microbiology & Fermentation Technology)
- Isolation and Characterization of Thermotolerant Gluconobacter Strains Catalyzing Oxidative Fermentation at Higher Temperatures
- Molecular Target of Piperine in the Inhibition of Lipid Droplet Accumulation in Macrophages(Biochemistry)
- Molecular Properties of Membrane-Bound FAD-Containing D-Sorbitol Dehydrogenase from Thermotolerant Gluconobacter frateurii Isolated from Thailand
- 3-Dehydroquinate Production by Oxidative Fermentation and Further Conversion of 3-Dehydroquinate to the Intermediates in the Shikimate Pathway(Microbiology & Fermentation Technology)
- Purification and Characterization of Membrane-bound Quinoprotein Quinate Dehydrogenase(Biochemistry & Molecular Biology)
- Molecular Cloning of Quinohemoprotein Alcohol Dehydrogenase, ADH IIB, from Pseudomonas putida HK5(Biochemistry & Molecular Biology)
- Membrane-bound Quinoprotein D-Arabitol Dehydrogenase of Gluconobacter suboxydans IFO 3257 : A Versatile Enzyme for the Oxidative Fermentation of Various Ketoses(Microbiology & Fermentation Technology)
- Total Synthesis of Malformin C, an Inhibitor of Bleomycin-Induced G2 Arrest
- 1S-Ep05 耐熱性酢酸菌を使った酸化発酵による有用物質生産系の開発(伝統的醗酵微生物の新しい利用展開,シンポジウム,伝統の技と先端科学技術の融合)
- Biochemical and Spectroscopic Properties of Cyanide-Insensitive Quinol Oxidase from Gluconobacter oxydans
- Guadinomines, Type III Secretion System Inhibitors, Produced by Streptomyces sp. K01-0509 : II. Physico-chemical Properties and Structure Elucidation
- Guadinomines, Type III Secretion System Inhibitors, Produced by Streptomyces sp. K01-0509 : I. Taxonomy, Fermentation, Isolation and Biological Properties
- 琉球大学農学部「亜熱帯生物資源科学科,発酵・生命科学コース」と,設置予定の「泡盛学寄附講座」の紹介(九州支部,Branch Spirit)
- 2P030 酢酸菌Gluconobacter frateurii由来L-sorbose reductaseのX線結晶構造解析(蛋白質-構造機能相関,第48回日本生物物理学会年会)
- A Novel Polysaccharide Involved in the Pellicle Formation of Acetobacter aceti
- Citrinamides, New Potentiators of Antifungal Miconazole Activity, Produced by Penicillium sp. FKI-1938
- Tensyuic Acids, New Antibiotics Produced by Aspergillus niger FKI-2342
- An Ideal Basal Medium for Assaying Growth Stimulating Activity of Pyrroloquinoline Quinone with Acetobacter aceti IFO 3284(Microbiology & Fermentation Industry)
- An Enzyme Catalyzing Liberation of Pyrroloquinoline Quinone (PQQ) from PQQ-Adducts(Microbiology & Fermentation Industry)
- Anti-atherosclerotic Activity of Triacsin C, an Acyl-CoA Synthetase Inhibitor
- The Selectivity of Beauveriolide Derivatives in Inhibition toward the Two Isozymes of Acyl-CoA: cholesterol Acyltransferase
- Selective Inhibition of Acyl-CoA : cholesterol Acyltransferase 2 Isozyme by Flavasperone and Sterigmatocystin from Aspergillus Species
- Selectivity of Pyripyropene Derivatives in Inhibition toward Acyl-CoA : cholesterol Acyltransferase 2 Isozyme
- Characterization of Membrane-bound Spermidine Dehydrogenase of Citrobacter freundii
- Quinoprotein Alcohol Dehydrogenase of Acetic Acid Bacteria: Kinetic Study on the Enzyme Purified from Acetobacter methanolicus
- Cyslabdan, a New Potentiator of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Streptomyces sp. K04-0144 : II. Biological Activities
- Cyslabdan, a New Potentiator of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Streptomyces sp. K04-0144 : I. Taxonomy, Fermentation, Isolation and Structural Elucidation
- Enzymes Responsible for Acetate Oxidation by Acetic Acid Bacteria
- Microbiological Aspects of Acetate Oxidation by Acetic Acid Bacteria, Unfavorable Phenomena in Vinegar Fermentation
- Inhibition of Lipid Droplet Accumulation in Mouse Macrophages by Stemphone Derivatives
- Structure-activity Relationships of Stemphones, Potentiators of Imipenem Activity against Methicillin-resistant Staphylococcus aureus
- Purification and Characterization of Methylamine Oxidase Induced in Aspergillus niger AKU 3302
- NADPH Oxidase System as a Superoxide-generating Cyanide-Resistant Pathway in the Respiratory Chain of Corynebacterium glutamicum
- Role of Cytochrome c-553(CO), the Second Subunit of Alcohol Dehydrogenase, in the Azide-Insensitive Respiratory Chain and in Oxidative Fermentation of Gluconobacter Species
- Ethanol Oxidase Respiratory Chain of Acetic Acid Bacteria. Reactivity with Ubiquinone of Pyrroloquinoline Quinone-dependent Alcohol Dehydrogenases Purified from Acetobacter aceti and Gluconobacter suboxydans
- Formaldehyde Elimination with Formaldehyde and Formate Oxidase in Membrane of Acetic Acid Bacteria(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Involvement of Multiple Copper/Topa Quinone-Contanining and Flavin-Containing Amine Oxidases and NAD(P)^+ Aldehyde Dehydrogenases in Amine Degradation by Filamentous Fungi
- Azurin Involved in Alcohol Oxidation System in Pseudomonas putida HK5 : Expression Analysis and Gene Cloning(Biochemistry & Molecular Biology)
- Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway
- Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production
- High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria
- Conversion of Capsular Polysaccharide, Involved in Pellicle Formation, to Extracellular Polysaccharide by galE Deletion in Acetobacter tropicalis
- Growth Promoting Substance in Yeast Extract for Methylotrophic Growth of Candida boidinii
- A Novel Type of Formaldehyde-Oxidizing Enzyme from the Membrane of Acetobacter sp. SKU 14
- Electron Transfer Ability from NADH to Menaquinone and from NADPH to Oxygen of Type II NADH Dehydrogenase of Corynebacterium glutamicum
- Quinoprotein Alcohol Dehydrogenase Is Involved in Catabolic Acetate Production, while NAD-Dependent Alcohol Dehydrogenase in Ethanol Assimilation in Acetobacter pasteurianus SKU1108(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Purification and Characterization of Two NAD-Dependent Alcohol Dehydrogenases (ADHs) Induced in the Quinoprotein ADH-Deficient Mutant of Acetobacter pasteurianus SKU1108(Microbiology & Fermentation Technology)
- Purification and Characterization of a Novel Polysaccharide Involved in the Pellicle Produced by a Thermotolerant Acetobacter Strain(Microbiology & Fermentation Technology)
- Purification and Characterization of Membrane-bound Malate Dehydrogenase from Acetobacter sp. SKU 14(Microbiology & Fermentation Technology)
- Membrane-bound Sugar Alcohol Dehydrogenase in Acetic Acid Bacteria catalyzes L-Ribulose Formation and NAD-Dependent Ribitol Dehydrogenase is Independent of the Oxidative Fermentation
- Crystallization and Properties of NADPH-Dependent L-Sorbose Reductase from Gluconobacter melanogenus IFO 3294
- Crystallization and Properties of NAD-Dependent D-Sorbitol Dehydrogenase from Gluconobacter suboxydans IFO 3257
- Development of Thermotolerant Acetic Acid Bacteria Useful for Vinegar Fermentation at Higher Temperatures
- A Tightly Bound Quinone Functions in the Ubiquinone Reaction Sites of Quinoprotein Alcohol Dehydrogenase of an Acetic Acid Bacterium, Gluconobacter suboxydans
- A Novel 3-Dehydroquinate Dehydratase Catalyzing Extracellular Formation of 3-Dehydroshikimate by Oxidative Fermentation of Gluconobacter oxydans IFO 3244
- Energy Metabolism of a Unique Acetic Acid Bacterium, Asaia bogorensis, That Lacks Ethanol Oxidation Activity
- Distinct Physiological Roles of Two Membrane-Bound Dehydrogenases Responsible for D-Sorbitol Oxidation in Gluconobacter frateurii
- Preparation of Enzymes Required for Enzymatic Quantification of 5-Keto-D-gluconate and 2-Keto-D-gluconate
- Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
- 耐熱性酢酸菌を使った酸化発酵による有用物質生産系の開発(伝統的発酵微生物の新しい利用展開)
- Characterization of Quinohemoprotein Amine Dehydrogenase from Pseudomonas putida
- Soluble and Membrane-bound Quinoprotein D-Glucose Dehydrogenases of the Acinetobacter calcoaceticus : The Binding Process of PQQ to the Apoenzymes
- 6.ピロロキノリンキノン(PQQ)高生産菌Methylobacillus glycogenesの再分類とPQQ生産条件の検討(第424回研究協議会研究発表要旨,ビタミンB研究委員会)
- Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
- Global Analysis of the Genes Involved in the Thermotolerance Mechanism of Thermotolerant Acetobacter tropicalis SKU1100
- Makomotindoline from Makomotake, Zizania latifolia infected with Ustilago esculenta
- 沖縄の伝統発酵食品と微生物 : 泡盛を中心に(伝統発酵食品研究の新展開〜微生物共生から探る〜)
- Chitinase inhibitors : extraction of the active framework from natural argifin and use of in situ click chemistry
- 沖縄とバイオ、泡盛を飲みながら
- Solid-phase total synthesis of the chitinase inhibitor Argadin using a supported acetal resin
- New verticilides, inhibitors of acyl-CoA : cholesterol acyltransferase, produced by Verticillium sp. FKI-2679
- New isochaetochromin, an inhibitor of triacylglycerol synthesis in mammalian cells, produced by Penicillium sp. FKI-4942 : II. structure elucidation
- New isochaetochromin, an inhibitor of triacylglycerol synthesis in mammalian cells, produced by Penicillium sp. FKI-4942 : I. Taxonomy, fermentation, isolation and biological properties
- The high mannose-type glycan binding lectin actinohivin : dimerization greatly improves anti-HIV activity
- Production of monapinones by fermentation of the dinapinone-producing fungus Penicillium pinophilum FKI-3864 in a seawater-containing medium
- Dinapinones, novel inhibitors of triacylglycerol synthesis in mammalian cells, produced by Penicillium pinophilum FKI-3864
- Spoxazomicins A-C, novle antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolinicum K07-0460^T
- Solid-phase synthesis and biological activity of malformin C and its derivatives
- Xanthoradones, new potentiators of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Penicillium radicum FKI-3765-2 : II. Structure elucidation
- Xanthoradones, new potentiators of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Penicillium radicum FKI-3765-2 : I. Taxonomy, fermentation, isolation and biological properties
- Pentacecilides, new inhibitors of lipid droplet formation in mouse macrophages produced by Penicillium cecidicola FKI-3765-1 : II. Structure elucidation
- Pentacecilides, new inhibitors of lipid droplet formation in mouse macrophages, produced by Penicillium cecidicola FKI-3765-1 : I. Taxonomy, fermentation, isolation and biological properties
- Expression of two human acyl-CoA : diacylglycerol acyltransferase isozymes in yeast and selectivity of microbial inhibitors toward the isozymes
- Total synthesis of amidepsine B and revision of its absolute configuration
- 最新ビタミン学講話 : ビタミン発見100周年を記念して(18)PQQ(ピロロキノリンキノン)
- Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae. II : Structure elucidation
- Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae I : Fermentation, isolation and biological properties
- Production of a new type of amidepsine with a sugar moiety by static fermentation of Humicola sp. FO-2942
- Absolute stereochemistry of pentacecilides, new inhibitors of lipid droplet formation in mouse macrophages, produced by Penicillium cecidicola FKI-3765-1
- Actinohivin : specific amino acid residues essential for anti-HIV activity
- Xanthoradone C, a new potentiator of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Penicillium radicum FKI-3765-2
- Calpinactam, a new anti-mycobacterial agent, produced by Mortierella alpina FKI-4905
- Verticilide, a new ryanodine-binding inhibitor, produced by Verticillium sp. FKI-1033
- Characterization of Genes Involved in D-Sorbitol Oxidation in Thermotolerant Gluconobacter frateurii
- 2Ca10 酢酸菌膜結合型ヘテロ三量体ソルビトール脱水素酵素の分子構築過程における小サブユニットの役割(酵素学,酵素工学,一般講演)
- 4Dp09 Gluconobacter frateuriiのソルビトール酸化に関与する遺伝子の特徴付け(発酵生理学,発酵工学,一般講演)
- Significance of microbial symbiotic coexistence in traditional fermentation