Preparation of Enzymes Required for Enzymatic Quantification of 5-Keto-D-gluconate and 2-Keto-D-gluconate

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概要

• 論文の詳細を見る

• For easy measurement of 5-keto D-gluconate (5KGA) and 2-keto D-gluconate (2KGA), two enzymes, 5KGA reductase (5KGR) and 2KGA reductase (2KGR) are useful. The gene for 5KGR has been reported, and a corresponding gene was found in the genome of Gluconobacter oxydans 621H and was identified as GOX2187. On the other hand, the gene for 2KGR was identified in this study as GOX0417 from the N-terminal amino acid sequence of the partially purified enzyme. Several plasmids were constructed to express GOX2187 and GOX0417, and the final constructed plasmids showed good expression of 5KGR and 2KGR in Escherichia coli. From the two E. coli transformants, large amounts of each enzyme were easily prepared after one column chromatography, and the preparation was ready to use for quantification of 5KGA or 2KGA.

• 社団法人 日本農芸化学会の論文
• 2007-10-23

著者

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agricultre, Yamaguchi University

• Toyama Hirohide

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Department Of Agricultural Chemistry Yamaguchi University

• Hirose T

  School Of Pharmacy Kitasato University

• Tanaka Y

  School Of Pharmaceutical Sciences Kitasato University The Kitasato Institute

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Tanaka H

  External Science Affairs Fujisawa Pharmaceutical Co. Ltd.

• Matsushita K

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• SAICHANA Ittipon

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• TOYAMA Hirohide

  Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University

• 松下 一信

  山口大 農

• Saichana Ittipon

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi O

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Ano Yoshitaka

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Applied Bioscience, Faculty of Agriculture, Ehime University

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