Vanilin Formation by Microbial Amine Oxidases from Vanillylamine
スポンサーリンク
概要
- 論文の詳細を見る
Vanillin is a main flavor ingredient of vanilla beans. It is widely used as a food flavor, and is an important material in various industrial fields. The production of vanillin from vanillylamine was investigated using amine oxidase (AO) from Aspergillus niger and monoamine oxidase (MAO) from Escherichia coli, which have previously been isolated, characterized, and molecularly cloned in our laboratory. Oxidation of vanillylamine was observed with both AO and MAO. Kinetic analyses of the enzyme reactions revealed that AO was a more efficient producer of vanillin than MAO. With AO, 1 mM of vanillylamine was oxidized almost completely in about 30 min by 2.7 μg/ml of the enzyme. The product of the enzyme reaction was confirmed to be vanillin by HPLC and GC-MS. Continuous production of vanillin with immobilized AO was also investigated. The results suggest that AO from A. niger will be useful for the industrial synthesis of vanillin.
- 社団法人日本生物工学会の論文
- 1997-12-25
著者
-
ADACHI Osao
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
-
Kumagai Hidehiko
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Adachi Osao
Department Of Agricultural Chemistry Yamaguchi University
-
TAMAKI HISANORI
Division of Integrated Life Sciences, Graduate School of Biostudies, Kyoto University
-
YOSHIDA AYA
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
TAKENAKA YASUHIRO
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
FREBORT IVO
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
-
Frebort Ivo
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
-
Tamaki Hisanori
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Takenaka Yasuhiro
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Yoshida Aya
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
関連論文
- L-Erythrulose Production by Oxidative Fermentation is Catalyzed by PQQ-Containing Membrane-bound Dehydrogenase(Microbiology & Fermentation Technology)
- Purification and Characterization of Membrane-bound Quinoprotein Cyclic Alcohol Dehydrogenase from Gluconobacter frateurii CHM 9(Microbiology & Fermentation Technology)
- Isolation and Characterization of Thermotolerant Gluconobacter Strains Catalyzing Oxidative Fermentation at Higher Temperatures
- Distinct Physiological Roles of Two Membrane-Bound Dehydrogenases Responsible for D-Sorbitol Oxidation in Gluconobacter frateurii
- Molecular Properties of Membrane-Bound FAD-Containing D-Sorbitol Dehydrogenase from Thermotolerant Gluconobacter frateurii Isolated from Thailand
- 3-Dehydroquinate Production by Oxidative Fermentation and Further Conversion of 3-Dehydroquinate to the Intermediates in the Shikimate Pathway(Microbiology & Fermentation Technology)
- Purification and Characterization of Membrane-bound Quinoprotein Quinate Dehydrogenase(Biochemistry & Molecular Biology)
- Molecular Cloning of Quinohemoprotein Alcohol Dehydrogenase, ADH IIB, from Pseudomonas putida HK5(Biochemistry & Molecular Biology)
- Membrane-bound Quinoprotein D-Arabitol Dehydrogenase of Gluconobacter suboxydans IFO 3257 : A Versatile Enzyme for the Oxidative Fermentation of Various Ketoses(Microbiology & Fermentation Technology)
- Methionaquinone is a Direct Natural Electron Acceptor for the Membrane-bound Hydrogenase in Hydrogenobacter thermophilus Strain TK-6(Biological Chemistry)
- Production of 4-Keto-D-arabonate by Oxidative Fermentation with Newly Isolated Gluconacetobacter liquefaciens
- A Tightly Bound Quinone Functions in the Ubiquinone Reaction Sites of Quinoprotein Alcohol Dehydrogenase of an Acetic Acid Bacterium, Gluconobacter suboxydans
- Preparation and Preservation of Freeze-dried Cells of Acetic Acid Bacteria with Aldehyde Oxidase Activity
- Cloning of a Gene Encoding a Highly Stable Endo-β-1, 4-Glucanase from Aspergillus niger and Its Expression in Yeast
- Transcriptional Regulation of Tyrosine Phenol-lyase Gene Mediated through TyrR and cAMP Receptor Protein
- Transglycosylation Activity of the Endo-β-1, 4-Glucanase from Aspergillus niger IFO31125 and Its Application
- Effects of Carbohydrate Chain on Surface Net Charge and Hydrophobicity of Glycoenzymes
- A Novel Polysaccharide Involved in the Pellicle Formation of Acetobacter aceti
- Purification and Characterization of Thermostable α-Galactosidase from Ganoderma lucidum(Biochemistry & Molecular Biology)
- Vanilin Formation by Microbial Amine Oxidases from Vanillylamine
- Purification and Characterization of Aminopeptidase B from Escherichia coli K-12(Microbiology & Fermentation Technology)
- Characterization of Membrane-bound Spermidine Dehydrogenase of Citrobacter freundii
- Solubilization, Purification, and Properties of Membrane-Bound D-Glucono-δ-lactone Hydrolase from Gluconobacter oxydans
- Quinoprotein Alcohol Dehydrogenase of Acetic Acid Bacteria: Kinetic Study on the Enzyme Purified from Acetobacter methanolicus
- Enzymes Responsible for Acetate Oxidation by Acetic Acid Bacteria
- Microbiological Aspects of Acetate Oxidation by Acetic Acid Bacteria, Unfavorable Phenomena in Vinegar Fermentation
- Purification and Characterization of Methylamine Oxidase Induced in Aspergillus niger AKU 3302
- NADPH Oxidase System as a Superoxide-generating Cyanide-Resistant Pathway in the Respiratory Chain of Corynebacterium glutamicum
- Tissue Localization of Cytokinin Dehydrogenase in Maize : Possible Involvement of Quinone Species Generated from Plant Phenolics by Other Enzymatic Systems in the Catalytic Reaction
- Role of Cytochrome c-553(CO), the Second Subunit of Alcohol Dehydrogenase, in the Azide-Insensitive Respiratory Chain and in Oxidative Fermentation of Gluconobacter Species
- Ethanol Oxidase Respiratory Chain of Acetic Acid Bacteria. Reactivity with Ubiquinone of Pyrroloquinoline Quinone-dependent Alcohol Dehydrogenases Purified from Acetobacter aceti and Gluconobacter suboxydans
- Formaldehyde Elimination with Formaldehyde and Formate Oxidase in Membrane of Acetic Acid Bacteria(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Energy Metabolism of a Unique Acetic Acid Bacterium, Asaia bogorensis, That Lacks Ethanol Oxidation Activity
- A Novel 3-Dehydroquinate Dehydratase Catalyzing Extracellular Formation of 3-Dehydroshikimate by Oxidative Fermentation of Gluconobacter oxydans IFO 3244
- Involvement of Multiple Copper/Topa Quinone-Contanining and Flavin-Containing Amine Oxidases and NAD(P)^+ Aldehyde Dehydrogenases in Amine Degradation by Filamentous Fungi
- Azurin Involved in Alcohol Oxidation System in Pseudomonas putida HK5 : Expression Analysis and Gene Cloning(Biochemistry & Molecular Biology)
- Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway
- Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production
- High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria
- Conversion of Capsular Polysaccharide, Involved in Pellicle Formation, to Extracellular Polysaccharide by galE Deletion in Acetobacter tropicalis
- Growth Promoting Substance in Yeast Extract for Methylotrophic Growth of Candida boidinii
- A Novel Type of Formaldehyde-Oxidizing Enzyme from the Membrane of Acetobacter sp. SKU 14
- Electron Transfer Ability from NADH to Menaquinone and from NADPH to Oxygen of Type II NADH Dehydrogenase of Corynebacterium glutamicum
- Acetic Acid Fermentation of Acetobacter pasteurianus: Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation
- Quinoprotein Alcohol Dehydrogenase Is Involved in Catabolic Acetate Production, while NAD-Dependent Alcohol Dehydrogenase in Ethanol Assimilation in Acetobacter pasteurianus SKU1108(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Purification and Characterization of Two NAD-Dependent Alcohol Dehydrogenases (ADHs) Induced in the Quinoprotein ADH-Deficient Mutant of Acetobacter pasteurianus SKU1108(Microbiology & Fermentation Technology)
- Purification and Characterization of a Novel Polysaccharide Involved in the Pellicle Produced by a Thermotolerant Acetobacter Strain(Microbiology & Fermentation Technology)
- Purification and Characterization of Membrane-bound Malate Dehydrogenase from Acetobacter sp. SKU 14(Microbiology & Fermentation Technology)
- Membrane-bound Sugar Alcohol Dehydrogenase in Acetic Acid Bacteria catalyzes L-Ribulose Formation and NAD-Dependent Ribitol Dehydrogenase is Independent of the Oxidative Fermentation
- Crystallization and Properties of NADPH-Dependent L-Sorbose Reductase from Gluconobacter melanogenus IFO 3294
- Crystallization and Properties of NAD-Dependent D-Sorbitol Dehydrogenase from Gluconobacter suboxydans IFO 3257
- Development of Thermotolerant Acetic Acid Bacteria Useful for Vinegar Fermentation at Higher Temperatures
- Further Properties of Spermidine Dehydrogenase from Citrobacter freundii IFO 12681
- A Tightly Bound Quinone Functions in the Ubiquinone Reaction Sites of Quinoprotein Alcohol Dehydrogenase of an Acetic Acid Bacterium, Gluconobacter suboxydans
- A Novel 3-Dehydroquinate Dehydratase Catalyzing Extracellular Formation of 3-Dehydroshikimate by Oxidative Fermentation of Gluconobacter oxydans IFO 3244
- Energy Metabolism of a Unique Acetic Acid Bacterium, Asaia bogorensis, That Lacks Ethanol Oxidation Activity
- Distinct Physiological Roles of Two Membrane-Bound Dehydrogenases Responsible for D-Sorbitol Oxidation in Gluconobacter frateurii
- Prosthetic Group of Aldehyde Dehydrogenase in Acetic Acid Bacteria Not Pyrroloquinoline Quinone
- Purification and Characterization of Membrane-Bound 3-Dehydroshikimate Dehydratase from Gluconobacter oxydans IFO 3244, A New Enzyme Catalyzing Extracellular Protocatechuate Formation
- Production of 4-Keto-D-arabonate by Oxidative Fermentation with Newly Isolated Gluconacetobacter liquefaciens
- Conversion of Quinate to 3-Dehydroshikimate by Ca-Alginate-Immobilized Membrane of Gluconobacter oxydans IFO 3244 and Subsequent Asymmetric Reduction of 3-Dehydroshikimate to Shikimate by Immobilized Cytoplasmic NADP-Shikimate Dehydrogenase
- Acetic Acid Fermentation of Acetobacter pasteurianus : Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation
- Solubilization, Purification, and Properties of Membrane-Bound D-Glucono-δ-lactone Hydrolase from Gluconobacter oxydans
- Preparation of Enzymes Required for Enzymatic Quantification of 5-Keto-D-gluconate and 2-Keto-D-gluconate
- Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
- Purification and Properties of Two Different Dihydroxyacetone Reductases in Gluconobacter suboxydans Grown on Glycerol
- The Occurrence of a Novel NADH Dehydrogenase, Distinct from the Old Yellow Enzyme, in Gluconobacter Strains
- Copper/Quinone-Containing Amine Oxidases, an Exciting Class of Ubiquitous Enzymes
- Soluble and Membrane-bound Quinoprotein D-Glucose Dehydrogenases of the Acinetobacter calcoaceticus : The Binding Process of PQQ to the Apoenzymes
- Monoclonal Antibody Recognizing the Quinoprotein Subunit of Alcohol Dehydrogenase Complex from Gluconobacter Species(Biological Chemistry)
- Formation of the Apo-form of Quinoprotein Alcohol Dehydrogenase from Gluconobacter suboxydans(Microbiology & Fermentation Industry)
- Sugar-oxidizing Respiratory Chain of Gluconobacter suboxydans. Evidence for a Branched Respiratory Chain and Characterization of Respiratory Chain-Linked Cytochromes(Microbiology & Fermentation Industry)
- Selective Production of 5-Keto-D-Gluconate by Gluconobacter Strains
- Formation of 4-Keto-D-aldopentoses and 4-Pentulosonates (4-Keto-D-pentonates) with Unidentified Membrane-Bound Enzymes from Acetic Acid Bacteria
- Effect of Growth Sunstrates on Formation of Alcohol Dehydrogenase in Acetobacter methanolicus and Acetobacter aceti
- Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
- Global Analysis of the Genes Involved in the Thermotolerance Mechanism of Thermotolerant Acetobacter tropicalis SKU1100
- Formation of 4-Keto-D-aldopentoses and 4-Pentulosonates (4-Keto-D-pentonates) with Unidentified Membrane-Bound Enzymes from Acetic Acid Bacteria
- Development of a heat-processing method for koji to enhance its antioxidant activity(BREWING AND FOOD TECHNOLOGY)
- Enzymatic Synthesis of 4-Pentulosonate (4-Keto-D-pentonate) from D-Aldopentose and D-Pentonate by Two Different Pathways Using Membrane Enzymes of Acetic Acid Bacteria
- Identification of the Prosthetic Group and Further Characterization of a Novel Enzyme, Polyethylene Glycol Dehydrogenase
- Pyrroloquinoline Quinone as an Essential Growth Factor for a Poly(vinyl alcohol)-degrading Symbiont, Pseudomonas sp. VM15C
- Development of a heat-processing method for koji to enhance its antioxidant activity
- Characterization of Genes Involved in D-Sorbitol Oxidation in Thermotolerant Gluconobacter frateurii
- Membrane-bound, Electron Transport-linked, D-Glucose Dehydrogenase of Pseudomonas fluorescens. Interaction of the Purified Enzyme with Ubiquinone or Phospholipid
- D-Gluconate Dehydrogenase, 2-Keto-D-gluconate Yielding, from Gluconobacter dioxyacetonicus: Purification and Characterizationc
- Pentose Oxidation by Acetic Acid Bacteria Led to a Finding of Membrane-Bound Purine Nucleosidase