Purification and Characterization of Thermostable α-Galactosidase from Ganoderma lucidum(Biochemistry & Molecular Biology)

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概要

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• α-Galactosidase was purified from a fresh fruiting body of Ganoderma lucidum by precipitation with ammonium sulfate and column chromatographies with DEAE-Sephadex and Con A-Sepharose. The purified enzyme was homogeneous on polyacrylamide gel electrophoresis. Its N-terminal amino acid sequence was similar to that of Mortierella vinacea α-galactosidase. The molecular mass of the enzyme was about 56 kDa by SDS-polyacrylamide gel electrophoresis, and about 249 kDa by gel filtration column chromatography. The optimum pH and temperature were 6.0 and 70℃, respectively. The enzyme was fully stable to heating at 70℃ for 30min. It hydrolyzed p-nitrophenyl-α-D-galactopyranoside (K_m=0.4 mM) but hydrolyzed little o-nitrophenyl-α-D-galactopyranoside. It also hydrolyzed melibiose, raffinose, and stachyose. The enzyme catalyzed the transgalactosylation reaction which synthesized melibiose. The product was confirmed by various analyses.

• 社団法人日本農芸化学会の論文
• 2003-07-23

著者

• Kumagai H

  Kyoto Univ. Kyoto Jpn

• Kumagai Hidehiko

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yamamoto Kenji

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• Sripuan Thida

  Department Of Chemistry Faculty Of Science Chiang Mai University:institute For Science And Technolog

• Aoki K

  Graduate School Of Biostudies Kyoto University

• Kumagai Hidehiko

  Faculty Of Agriculture Kyoto University

• AOKI Kazuhiro

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• TONGKAO Dararat

  Department of Chemistry, Faculty of Science, Chiang Mai University

• Tongkao Dararat

  Department Of Chemistry Faculty Of Science Chiang Mai University

• Kumagai Hidehiko

  Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.

• Kumagai Hidehiko

  Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University

• 山本 憲二

  Graduate School Of Biostudies Kyoto University

• 河合 弘康

  関西女子短期大学

• Kumagai H

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamamoto Kenji

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

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