Increase in β-D-Glucoside-assimilating Ability of Bifidobacterium breve 203 Due to Acclimation to β-D-Glucoside(Microbiology & Fermentation Industry)

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概要

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• bifidobacterium breve 203, an isolate which assimilates β-D-glucosides weakly, came to grow well on cellobiose (B. breve clb strain) or gentiobiose (B. breve gnt strain) after successive transfers in medium containing cellobiose or gentiobiose as a carbon source. The elevation of the assimilating ability of B. breve clb, which might reflect both higher cellobiose consuming-and β-D-glucosidase I activities than those of the original B. breve 203, was specific to cellobiose. In the case of B. breve gnt, the elevated assimilating ability was specific to gentiobiose, with higher gentiobiose consuming-and β-D-glucosidase II activities. Subsequent transfers (10 times) of B. breve clb in medium with glucose as a carbon source resulted only in a decrease in cellobiose consumption by the intact cells, while similar treatment of B. breve gnt resulted in decreases in both gentiobiose consumption and β-D-glucosidase II activity. β-D-Glucosidase I partially purified from B. breve clb grown on glucose as a carbon source showed the same properties as the enzyme from B. breve 203 in molecular weight, optimum pH for the reaction, effects of divalent cations and sulfhydryl reagents, substrate specificity, β-D-fucoside-transferring activity and so on.

• 社団法人日本農芸化学会の論文
• 1987-03-22

著者

• Sakai Kenji

  Department of Plant Resources, Kyushu University

• TACHIKI TAKASHI

  Department of Food Science and Technology, Kyoto University

• KUMAGAI HIDEHIKO

  Department of Food Science and Technology, Kyoto University

• TOCHIKURA TATSUROKURO

  Department of Food Science and Technology, Kyoto University

• Kumagai Hidehiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Faculty Of Agriculture Kyoto University

• TANAKA Masaru

  Department of Cardiology, Cardiovascular Center, Osaka Red Cross Hospital

• Tochikura Tatsurokuro

  Department Of Agricultural Chemistry Kyoto University

• Tachiki Takashi

  Department Of Chemistry Ritsumeikan University

• Sakai Kenji

  Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University

• Kumagai H

  Department Of Agricultural Chemistry The University Of Tokyo

• Tachiki Takashi

  Faculty Of Home Economics Kobe Women's University

• Tachiki Takashi

  Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit

• Tanaka Masaru

  Department Of Cardiology Cardiovascular Center Osaka Red Cross Hospital

• Tanaka Masaru

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Sakai Kenji

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• KUMAGAI Hidehiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

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