Molecular Cloning and Determination of the Nucleotide Sequence of a Gene Encoding Salt-Tolerant Glutaminase from Micrococcus luteus K-3

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概要

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• The gene encoding the salt-tolerant glutaminase I from marine Micrococcus luteus K-3 (M. luteus K-3) was cloned in Escheria coli (E. coli) JM109. Clones were screened by hybridization with degenerate oligonucleotide probes designed using the known N-terminal amino acid sequence of glutaminase I from M. luteus K-3. A 2.4-kb HincII fragment from a 8-kb primary cloned DNA fragment was subcloned and sequenced. This fragment had an open reading frame of 1,368 nucleotides encoding 456 amino acids. The molecular weight of the deduced amino acid sequence of glutaminase I was determined to be 48,247. Comparison of the amino acid sequence of glutaminase I with those of kidney, brain, liver, and Caenorhabditis elegans glutaminases revealed high degrees of homology in several local regions.

• 社団法人日本生物工学会の論文
• 1996-12-25

著者

• 酒井 謙二

  九大院生資環

• Sakai Kenji

  Department of Plant Resources, Kyushu University

• WAKAYAMA Mamoru

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• MORIGUCHI Mitsuaki

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• Nagano Y

  Pharmaceutical Research Division Takeda Chemical Industries Ltd.

• Wakayama Mamoru

  Dep. Of Biotechnology Fac. Of Life Sciences Ritsumeikan Univ.

• Wakayama Mamoru

  Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University

• Wakayama Mamoru

  Institute. For Chemical Research Kyoto University

• Moriguchi Mitsuaki

  Department Of Food And Bioscience Beppu University

• Moriguchi Mitsuaki

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Mori Masatsugu

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Sakai Kenji

  Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University

• NAGANO YOSHIO

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• RENU NANDAKUMAR

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• KAWAMURA TATSURO

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• Renu Nandakumar

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Nagano Yoshio

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• SAKAI Kenji

  Research Institute for Theoretical Physics Hiroshima University

• Kawamura Tatsuro

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Sakai Kenji

  Department Of Drug Metabolism And Pharmacokinetics Nippon Boehringer Ingelheim Co. Ltd.

• Sakai Kenji

  Department Of Applied Chemistry Faculty Of Engineering Oita University

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