Production, Purification, and Characterization of D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6

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概要

• 論文の詳細を見る

• The best inducers for D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6(Alcaligenes A-6) were a poor substrate, N-acetyl-γ-methyl-D-leucine, and an inhibitor, N-acetyl-D-alloisoleucine. The enzyme has been homogeneously purified. The molecular weight of the native enzyme was estimated to be 58,000 by gel filtration. A subunit molecular weight of 52,000 was measured by SDS-PAGE, indicating that the native protein is a monomer. The isoelectric point was 5.2. The enzyme was specific to the D-isomer and hydrolyzed N-acetyl derivatives of D-leucine, D-phenylalanine, D-norleucine, D-methionine, and D-valine, and also N-formyl, N-butyryl, and N-propionyl derivatives of D-leucine. The K_m for N-acetyl-D-leucine was 9.8 mM. The optimum pH and temperature were 7.0 and 50℃, respectively. The stabilities of pH and temperature were 8.1 and 40℃. D-Aminoacylases from three species of the genus Alcaligenes differ in inducer and substrate specificities, but are similar with respect to molecular weight and N-terminal amino acid sequence.

• 社団法人日本農芸化学会の論文
• 1993-07-23

著者

• 酒井 謙二

  九大院生資環

• Sakai Kenji

  Department of Plant Resources, Kyushu University

• WAKAYAMA Mamoru

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• Miura Y

  Department Of Applied Biological Science Tokyo Noko University

• Miura Yutaka

  Applied Bioresearch Center Kirin Brewry Co. Ltd.

• Miura Yutaka

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• MORIGUCHI Mitsuaki

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• Miyamoto Yoshiro

  Department Of Biochemical Engineering Faculty Of Computer Science And System Engineering Kyushu Inst

• Wakayama Mamoru

  Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University

• Moriguchi Mitsuaki

  Department Of Food And Bioscience Beppu University

• Moriguchi Mitsuaki

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Sakai Kenji

  Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University

• SAKAI Kenji

  Research Institute for Theoretical Physics Hiroshima University

• Sakai Kenji

  Department Of Drug Metabolism And Pharmacokinetics Nippon Boehringer Ingelheim Co. Ltd.

• Miyamoto Yoko

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Sakai Kenji

  Department Of Applied Chemistry Faculty Of Engineering Oita University

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