Isolation and Characterization of Two β-D-Glucosidases from Bifidobacterium breve 203(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
Two β-D-glucosidases were purified to homogeneity from Bifidobacterium breve 203: one (β-D-glucosidase I; molecular weight, 96,000) showed reactivity toward p-nitrophenyl (p-NP) β-D-fucoside, 74% of that to p-NP β-D-glucoside, and the other (β-D-glucosidase II; molecular weight, 450,000) did not. They also differed in their thermal and pH stabilities. Laminaribiose, cellobiose and gentiobiose were hydrolyzed by β-D-glucosidase I, with 53%, 34% and 3% of the reactivity in the case of p-NP β-D-glucoside, and by β-D-glucosidase II, with 53%, 6% and 107% of the reactivity. The reaction of β-D-glucosidase I with p-NP β-D-fucoside was enhanced by the addition of glucose and other monosaccharides to the reaction mixture, whereas that with p-NP β-D-glucoside was not affected. The activity of β-D-glucosidase II with p-NP β-D-glucoside was inhibited by glucose.
- 社団法人日本農芸化学会の論文
- 1986-09-23
著者
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Sakai Kenji
Department of Plant Resources, Kyushu University
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TACHIKI TAKASHI
Department of Food Science and Technology, Kyoto University
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KUMAGAI HIDEHIKO
Department of Food Science and Technology, Kyoto University
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TOCHIKURA TATSUROKURO
Department of Food Science and Technology, Kyoto University
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Kumagai Hidehiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Kumagai Hidehiko
Faculty Of Agriculture Kyoto University
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Tochikura Tatsurokuro
Department Of Agricultural Chemistry Kyoto University
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Tachiki Takashi
Department Of Chemistry Ritsumeikan University
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Sakai Kenji
Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University
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Kumagai H
Department Of Agricultural Chemistry The University Of Tokyo
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Tachiki Takashi
Faculty Of Home Economics Kobe Women's University
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Tachiki Tkashi
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Tachiki Takashi
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Sakai Kenji
Department Of Applied Chemistry Faculty Of Engineering Oita University
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