Role of Chitin Binding Domain of Chitinase A of Streptomyces cyaneus SP-27 in Protoplast Formation from Schizophyllum commune

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概要

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• Chitinase A (CHIA) of Streptomyces cyaneus SP-27 forms protoplasts from Schizophyllum commune mycelia when it is combined with α-1,3-glucanase of Bacillus circulans KA-304. An N-terminal chitin-binding domain truncated mutant (CatCHIA), which was expressed in Escherichia coli Rosetta-gami B (DE 3), lost most of its colloidal chitin- and powder chitin-binding activity. The colloidal chitin-hydrolyzing, the powder chitin-hydrolyzing, and the protoplast-forming activities of CatCHIA were lower than those of CHIA, suggesting that the chitin-binding domain contributes to the hydrolysis of chitin in the cell-wall of S. commune.

• 社団法人 日本農芸化学会の論文
• 2009-03-23

著者

• TACHIKI TAKASHI

  Department of Food Science and Technology, Kyoto University

• YANO Shigekazu

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• HONDA Arata

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• RATTANAKIT-CHANDET Nopakarn

  Department of Chemistry, Faculty of Science, Chiang Mai University

• WAKAYAMA Mamoru

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• Wakayama Mamoru

  Dep. Of Biotechnology Fac. Of Life Sciences Ritsumeikan Univ.

• Wakayama Mamoru

  Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University

• Tachiki T

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Tachiki Takashi

  Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit

• Yano Shigekazu

  Department Of Biotechnology College Of Life Sciences Ritsumeikan University

• Rattanakit Nopakarn

  Department Of Chemistry Faculty Of Science Chiang Mai University

• NODA Yuta

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• PLIKOMOL Abhinya

  Department of Biology, Faculty of Science, Chiang Mai University

• Yano Shuntaro

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Honda Arata

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Wakayama Mamoru

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Noda Yuta

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Plikomol Abhinya

  Department Of Biology Faculty Of Science Chiang Mai University

• YANO Shigekazu

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• Wakayama Mamoru

  Department of Applied Chemistry, Faculty of Engineering, Oita University

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