Occurrence of a Specific Protein in Basidiomycete-lytic Enzyme Preparation Produced by Bacillus circulans KA-304 Inductively with a Cell-wall Preparation of Schizophyllum commune(Microbiology & Fermentation Technology)
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概要
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KA-prep, a culture filtrate of Bacillus circulans KA-304 grown on a cell-wall preparation (CWP) of Schizophyllum commune, has been reported to have an activity to form protoplasts from S. commune mycelia. The SDS-polyacrylamide gel electrophoreses described here demonstrated that a specific proteinous component (molecular weight : 150,000) occurred in KA-prep. The protein (P150T) was also formed in culture filtrates with CWP of several basidiomycetes, which could release the protoplasts, suggesting that the component was an indispensable factor for protoplast formation. P150T, isolated from an ammonium sulfate fraction of KA-prep (0-30% saturation), did not have any protoplast-forming activity. Results were obtained indicating that P150T participates in protoplast formation together with chitinase (s) and β-glucanase (s) in KA-prep. The N-terminal amino acid sequence indicated an analogy of P150T to mutanase (α-1, 3-glucanase) from Bacillus sp. RM1, and actually P150T hydrolyzed mutan as well as S-(α-1, 3) glucan from S. commune.
- 社団法人日本農芸化学会の論文
- 2003-09-23
著者
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TACHIKI TAKASHI
Department of Food Science and Technology, Kyoto University
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YANO Shigekazu
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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WAKAYAMA Mamoru
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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Wakayama Mamoru
Dep. Of Biotechnology Fac. Of Life Sciences Ritsumeikan Univ.
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Wakayama Mamoru
Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University
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Wakayama Mamoru
Institute. For Chemical Research Kyoto University
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Yamamoto Sachiko
Department of Bioscience and Bioengineering, Ritsumeikan University
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TOGE Toshihiko
Department of Bioscience and Biotechnology, Faculty of Science and Technology, Ritsumeikan Universit
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Tachiki T
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Toge Toshihiko
Department Of Bioscience And Biotechnology Faculty Of Science And Technology Ritsumeikan University
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Tachiki Takashi
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Yano Shigekazu
Department Of Biotechnology College Of Life Sciences Ritsumeikan University
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Yano Shuntaro
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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Wakayama Mamoru
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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YANO Shigekazu
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University
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Wakayama Mamoru
Department of Applied Chemistry, Faculty of Engineering, Oita University
関連論文
- N-Terminal Region of Chitinase I of Bacillus circulans KA-304 Contained New Chitin-Biding Domain
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- Cloning and Expression of a Bacillus circulans KA-304 Gene Encoding Chitinase I, Which Participates in Protoplast Formation of Schizophyllum commune
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