Purification and Characterization of Nitrite-oxidizing Enzyme from Heterotrophic Bacillus badius I-73,with Special Concern to Catalase

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概要

• 論文の詳細を見る

• Nitrite-oxidizing enzyme I (NiOx I) was purified from a heterotrophic bacterium, Bacillus badius I-73. The enzyme was a homotetramer of a heme-containing peptide, and was similar to catalases from various sources in its N-terminal amino acid sequence. The purified enzyme also catalyzed H_2O_2 degradation. The nitrite oxidation reaction required ascorbic acid and oxygen. Successive H_2O_2 feeding could be substituted for ascorbic acid. These indicated that NiOx I is a catalase and nitrite was oxidized by a peroxidase-like reaction.

• 社団法人日本農芸化学会の論文
• 2000-12-23

著者

• 酒井 謙二

  九大院生資環

• 酒井 謙二

  九州大学大学院農学研究院

• Sakai Kenji

  Department of Plant Resources, Kyushu University

• WAKAYAMA Mamoru

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• NISIJIMA Hiroto

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• IKENAGA Yoshihito

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• MORIGUCHI Mitsuaki

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• Wakayama Mamoru

  Dep. Of Biotechnology Fac. Of Life Sciences Ritsumeikan Univ.

• Wakayama Mamoru

  Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University

• Wakayama Mamoru

  Institute. For Chemical Research Kyoto University

• Moriguchi Mitsuaki

  Department Of Food And Bioscience Beppu University

• Moriguchi Mitsuaki

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Sakai Kenji

  Department Of Physics Nagoya University

• Mori Masatsugu

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Sakai Kenji

  Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University

• SAKAI Kenji

  Research Institute for Theoretical Physics Hiroshima University

• Ikenaga Yoshihito

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Nisijima Hiroto

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Sakai Kenji

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Wakayama Mamoru

  Department of Applied Chemistry, Faculty of Engineering, Oita University

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