Isolation, Enzyme Production and Characterization of D-Aspartate Oxidase from Fusarium sacchari var. elongatum Y-105
スポンサーリンク
概要
- 論文の詳細を見る
A microorganism that produces D-aspartate-oxidizing enzyme by induction was isolated from soil, and identified as Fusarium sacchari var. elongatum Y-105. The enzyme catalyzed the oxidative deamination of D-aspartate ( _D-Asp) adn produced oxaloacetate, ammonia, and hydrogen peroxide, stoichiometrically. The enzyme is designated "D-Asp oxidase" (EC 1.4.3.1). In addition to D-Asp, the enzyme oxidized D-glutamate (D-Glu) and N-methyl-D-aspartate (NMDA). N-Acetyl-D-Asp and other D- or L-amino acids, however, were inert as substrates. The optimum pH and temperature were 7.5 and 40℃, respectively. The enzyme was stable at pH 9.0 and temperature of 50℃, respectively. The enzyme activity was not inhibited by sodium benzoate which is a specific inhibitor of D-amino acid oxidase from mammals. The Enzyme activity was also not affected by carboxylates such as meso- or D-tartarate, citrate, and fumarate which inhibit D-Asp oxidase from rabbits.
- 社団法人日本生物工学会の論文
- 1994-11-25
著者
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酒井 謙二
九大院生資環
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Sakai Kenji
Department of Plant Resources, Kyushu University
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WAKAYAMA Mamoru
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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MORIGUCHI Mitsuaki
Department of Applied Chemistry, Faculty of Engineering, Oita University
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Wakayama Mamoru
Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University
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Moriguchi Mitsuaki
Department Of Food And Bioscience Beppu University
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Moriguchi Mitsuaki
Department Of Applied Chemistry Faculty Of Engineering Oita University
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Mori Masatsugu
Department Of Applied Chemistry Faculty Of Engineering Oita University
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Sakai Kenji
Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University
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NAKASHIMA SADATOSHI
Department of Applied Chemistry, Faculty of Engineering, Oita University
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SAKAI Kenji
Research Institute for Theoretical Physics Hiroshima University
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Nakashima S
Gifu Univ. Gifu Jpn
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Sakai Kenji
Department Of Applied Chemistry Faculty Of Engineering Oita University
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