N-Terminal Region of Chitinase I of Bacillus circulans KA-304 Contained New Chitin-Biding Domain
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概要
- 論文の詳細を見る
Chitinase I (CHI1) of Bacillus circulans KA-304 forms protoplasts from Schizophyllum commune mycelia when the enzyme is combined with α-1,3-glucanase of B. circulans KA-304. CHI1 consists of an N-terminal unknown region and a C-terminal catalytic region classified into the glycoside hydrolase family-19 type. An N-terminal region-truncated mutant of CHI 1 (CatCHI1), which was expressed in Escherichia coli Rosetta-gami B (DE3), lost colloidal chitin- and powder chitin-binding activities. The colloidal chitin- and the powder chitin-hydrolyzing activities of CatCHI1 were lower than those of CHI1, and CatCHI1 was not effective in forming the protoplast. A fusion protein of the N-terminal region of CHI1 and green fluorescent protein (Nterm-GFP) was expressed in E. coli, and the fusion protein was adsorbed to colloidal chitin, powder chitin, and chitosan. Fluorescence microscopy analysis showed that Nterm-GFP bound to the S. commune cell-wall.
- 社団法人 日本農芸化学会の論文
- 2011-02-23
著者
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Takagi Kazuyoshi
Department Of Surgery Kurume University School Of Medicine
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TACHIKI TAKASHI
Department of Food Science and Technology, Kyoto University
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YANO Shigekazu
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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SUYOTHA Wasana
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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HONDA Arata
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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RATTANAKIT-CHANDET Nopakarn
Department of Chemistry, Faculty of Science, Chiang Mai University
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WAKAYAMA Mamoru
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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Wakayama Mamoru
Dep. Of Biotechnology Fac. Of Life Sciences Ritsumeikan Univ.
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Wakayama Mamoru
Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University
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Tachiki T
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Tachiki Takashi
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Yano Shigekazu
Department Of Biotechnology College Of Life Sciences Ritsumeikan University
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Rattanakit Nopakarn
Department Of Chemistry Faculty Of Science Chiang Mai University
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Yano Shuntaro
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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Takagi Kazuyoshi
Department Of Applied Chemistry Faculty Of Life Sciences Ritsumeikan University
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Honda Arata
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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Wakayama Mamoru
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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Suyotha Wasana
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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YANO Shigekazu
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University
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Wakayama Mamoru
Department of Applied Chemistry, Faculty of Engineering, Oita University
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