Production of Deoxyribonucleoside Triphosphates through Coupled Fermentation with Energy Transfer(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
The production of deoxyribonucleoside triphosphates (dNTPs) from deoxyribonucleoside monophosphates (dNMPs) was investigated using dried baker's yeast in the presence of a high concentration of inorganic phosphate. The amount of dATP formed by 150mg/ml of yeast cells was 127mM, with a 84% yield of the substrate, dAMP, after 18hr incubation at 28℃ with 150mM dAMP, 800mM glucose, 800mM potassium phosphate buffer (pH 7.0) and 10mM MgSO_4. The amount of dGTP or dCTP formed was in the range of 26〜34mM, from 100mM of either dGMP or dCMP, respectively, after 8 hr incubation, but dTTP was not formed during the incubation period. In a reaction mixture containing a dNMP-mixture, which was prepared through the hydrolysis of salmon testes DNA or herring sperm DNA (100mg/ml) with Nuclease P_1, 32〜49mM dNTP was obtained.
- 社団法人日本農芸化学会の論文
- 1989-07-23
著者
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YANO Toshihiro
Department of Material Science, Graduate School of Science, Osaka City University
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TACHIKI TAKASHI
Department of Food Science and Technology, Kyoto University
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KUMAGAI HIDEHIKO
Department of Food Science and Technology, Kyoto University
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TOCHIKURA TATSUROKURO
Department of Food Science and Technology, Kyoto University
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Kumagai H
Kyoto Univ.
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Kumagai Hidehiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Kumagai Hidehiko
Faculty Of Agriculture Kyoto University
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YASOHARA Yoshihiko
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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KASHIHARA Masaki
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Yasohara Yoshihiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Kumagai H
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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Yokoyama Tatsuo
Institute For Fermentation Osaka
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Tochikura Tatsurokuro
Faculty Of Home Economics Kobe Women's University
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Tochikura Tatsurokuro
Department Of Agricultural Chemistry Kyoto University
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Tachiki Takashi
Department Of Chemistry Ritsumeikan University
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Yano Toshihiro
Department Of Food Science Ishikawa Agricultural College
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Kumagai H
Department Of Agricultural Chemistry The University Of Tokyo
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Tachiki Takashi
Faculty Of Home Economics Kobe Women's University
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Tachiki Tkashi
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Tachiki Takashi
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Yano T
Research Laboratory Of Higashimaru Shoyu Co. Ltd.
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Kashihara Masaki
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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KUMAGAI Hidehiko
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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YANO Toshihiro
Department of Food Science and Technology, Kyoto University
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YANO Toshihiro
Department of Applied Chemistry, Nagoya Institute of Technology
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