Utilization of Shrimp Shellfish Waste as a Substrate for Solid-State Cultivation of Aspergillus sp. S1-13 : Evaluation of a Culture Based on Chitinase Formation Which Is Necessary for Chitin-Assimilation

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概要

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• The utilization of shrimp shellfish waste as a substrate for solid-state cultivation of a filamentous fungus, Aspergillus sp. S1-13, was investigated. The organism was selected from among 220 isolates based on the productivity of its chitinolytic enzyme (chitinase), which might reflect microbial growth. The enzyme was produced only when the organism was grown on medium containing the shellfish waste. The addition of 58-65% water (w/w) to the medium was effective in enhancing production, and a certain amount of enzyme was observed in media of higher water content (up to about 75%). The initial pH and nitrogen source (ammonium sulfate) of the solid-state medium also affected the amount of enzyme. The amount of enzyme increased 2-fold in an optimum solidstate medium: 5g of shrimp shellfish waste and 3ml of basal medium (pH 5) containing 0.1% (NH_4)_2SO_4 was inoculated with 4ml of spore suspension; static cultivation at room temperature. The amount increased further (1.5-fold) when the cultivation was carried out at 37℃, with 1.85 units of the enzyme formed from 1g of shrimp shellfish waste. An analysis by ion-exchange column chromatography suggested the presence of at least two colloidal chitin-hydrolyzing enzymes and one p-nitrophenyl β-D-N-acetylglucosaminide-hydrolyzing enzyme in an extract of the solidstate culture. The elution profile was similar to that obtained with a liquid culture filtrate.

• 社団法人日本生物工学会の論文
• 2002-06-20

著者

• TACHIKI TAKASHI

  Department of Food Science and Technology, Kyoto University

• YANO Shigekazu

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• WAKAYAMA Mamoru

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• Wakayama Mamoru

  Dep. Of Biotechnology Fac. Of Life Sciences Ritsumeikan Univ.

• Wakayama Mamoru

  Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University

• Rattanakit Nopakarn

  Department of Chemistry, Faculty of Science, Chiang Mai University

• Plikomol Abhinya

  Microbiology Section, Department of Biology, Faculty of Science, Chiang Mai University

• Tachiki T

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Tachiki Takashi

  Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit

• Yano Shigekazu

  Department Of Biotechnology College Of Life Sciences Ritsumeikan University

• Rattanakit Nopakarn

  Department Of Chemistry Faculty Of Science Chiang Mai University

• Yano Shuntaro

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Wakayama Mamoru

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Plikomol Abhinya

  Department Of Biology Faculty Of Science Chiang Mai University

• YANO Shigekazu

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• Wakayama Mamoru

  Department of Applied Chemistry, Faculty of Engineering, Oita University

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