Purification and Crystallization of Glutamine Synthetase from Gluconobacter suboxydans : Amino Acid Metablism in Acetic Acid Bacteria (X)
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概要
- 論文の詳細を見る
Glutamine synthetase was purified 530-fold with 2% yield from cell-free extracts of Gluconobacter suboxydans grown on glutamate as a sole nitrogen source. The purification procedure consisted of cell disruption, ammonium sulfate fractionation and chromatography on DEAE-cellulose, hydroxyapatite and Sephadex G-200. The purified enzyme was homogeneous on disc gel electrophoresis, and was obtained in a crysalline state by addition of ammonium sulfate. Some of the enzyme properties are described.
- 公益社団法人日本生物工学会の論文
- 1981-08-25
著者
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Tochikura Tatsurokuro
Department Of Food Science And Technology Kyoto University
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Tochikura Tatsurokuro
Department Of Agricultural Chemistry Kyoto University
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Tachiki Takashi
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Tachiki Takashi
Department Of Food Science And Technology Kyoto University
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