Glutamine Production by Coupling with Energy Transfer: Employing Yeast Cells and Gluconobacter Glutamine Synthetase
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概要
- 論文の詳細を見る
A glutamine production process was established by combining alcoholic fermentation of bakers yeast cells with glutamine synthetase from the bacterium Gluconobacter suboxydans. The maximumamount of glutamine formed under optimum conditions was about 20mMin 3 hr with 80% yield based on glutamate, substrate. The fermentation proceeded in two steps: the accumulation of energy in a form of fructose 1, 6-diphosphate (FDP) by yeast fermentation of sugar based on the Harden-Young effect and the fermentation of FDPcoupled with glutamine synthetase reaction (an endergonic reaction) through an ATP-ADPsystem. The following factors were found to be important: (a) the ratio of the activities of yeast fermentation of sugar and glutamine synthetase, (b) effect of contaminating enzyme(s) in glutamine synthetase preparation, and (c) enzymatic properties of glutamine synthetase.
- 社団法人 日本農芸化学会の論文
著者
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Tochikura Tatsurokuro
Department Of Agricultural Chemistry Kyoto University
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Tachiki Takashi
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Matsumoto Hiroko
Department Of Astronomy School Of Science Kyoto University
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YANO Toshihiro
Department of Food Science and Technology, Kyoto University
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MATSUMOTO Hiroko
Department of Food Science and Technology, Kyoto University
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