Repeated Batch Production of Theanine by Coupled Fermentation with Energy Transfer Using Membrane-Enclosed γ-Glutamylmethylamide Synthetase and Dried Yeast Cells

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概要

• 論文の詳細を見る

• γ-Glutamylmethylamide synthetase and dried baker’s yeast cells were enclosed together in a dialysis membrane tube to produce theanine repeatedly by coupled fermentation with energy transfer. The membrane-enclosed enzyme preparation (M-EEP) formed approximately 600 mM theanine from glutamic acid and ethylamine at a 100% conversion rate. M-EEP maintained its productivity of theanine during six consecutive reactions in a mixture containing NAD+.

• 社団法人 日本農芸化学会の論文
• 2009-12-23

著者

• TACHIKI TAKASHI

  Department of Food Science and Technology, Kyoto University

• YANO Shigekazu

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• WAKAYAMA Mamoru

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• Wakayama Mamoru

  Dep. Of Biotechnology Fac. Of Life Sciences Ritsumeikan Univ.

• Wakayama Mamoru

  Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University

• Wakayama Mamoru

  Institute. For Chemical Research Kyoto University

• Yamamoto Sachiko

  Department of Bioscience and Bioengineering, Ritsumeikan University

• MORIHARA Yosuke

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan Universi

• Tachiki T

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Tachiki Takashi

  Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit

• Yano Shuntaro

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Wakayama Mamoru

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Morihara Yosuke

  Department Of Biotechnology Faculty Of Life Science Ritsumeikan University

• Wakayama Mamoru

  Department of Applied Chemistry, Faculty of Engineering, Oita University

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