Production of ATP from Adenine by a Combination of Bacterial and Baker's Yeast Cells

概要

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A new process for ATP production from adenine was designed involving the use of intact bacterial cells containing nucleoside phosphorylases and dried baker's yeast cells containing an energy generating system : it consists of (1) the formation of adenosine through trans-N-ribosylation between adenine and uridine, cytidine, or inosine catalyzed by the nucleoside phosphorylase and (2) the phosphorylation of adenosine to ATP through alcoholic fermentation of the yeast cells. Erwinia carotovora was better than Escherishia coli or Aerobacter aerogenes as the source of nucleoside phosphorylases. Inosine and uridine were effective as ribosyl donors, and cytidine was also usable, if the reactions were done with cell-free extracts. ATP production was greatly influenced by the concentration of inorganic phosphate and glueose in the mixture. The immobilized phosphorylase preparation was better than the cell-free extract in the ATP production. The nucleoside phosphorylases were stable at 60℃ but adenosine deaminating enzymes were not, and large amounts of adenine could be used due to the increase in solubility at the high temperature. Through a two-step reaction, i.e., transribosylation with intact cells of Er. carotovora at 60℃ followed by fermentation with baker's yeast cells at 28℃, 98 mM ATP (about 50 g/l) was obtained from 150 mM adenine.
公益社団法人日本生物工学会の論文
1989-12-25