Purification and Characterization of β-D-Glucosidase (β-D-Fucosidase) from Bifidobacterium breve clb Acclimated to Cellobiose

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概要

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• The β-D-glucosidase (EC. 3.2.1.21) activity of Bifidobacterium breve 203 was increased by acclimation with cellobiose, and the enzyme was purified to homogeneity from cell-free extracts of an acclimatized strain of B. breve clb, by ammonium sulfate fractionation and column chromatographies of anion-exchange,gel filtration, Gigapaite, and hydrophobic interaction. This enzyme had not only β-D-glucosidase activity but also β-D-fucosidase activity, which is specific to Bifidobacteria in intestinal flora. The molecular weight of the purified enzyme was estimated to be 47,000-48,000 and the enzyme was assumed to be a monomeric protein. The optimum pH and temperature of the enzyme were around 5.5 and 45℃, respectively. The enzyme was stable up to 40℃ and between pH 5 and 8. The isoelectric point of the enzyme was 4.3 and the K_m values for p-nitrophenyl-β-D-glucoside and p-nitrophenyl-β-D-fucoside were 1.3 mM and 0.7 mM, respectively. This enzyme had also transferase activity for the β-D-fucosyl group but not for the β-D-glucosyl group. The N-terminal amino acid sequence of this enzyme was similar to those of β-D-glucosidase from other bacteria, actinomycetes, and plants.

• 社団法人日本農芸化学会の論文
• 1996-02-23

著者

• YAMAMOTO Kenji

  Department of Medical Ecology and Informatics, Research Institute, International Medical Center of J

• OHDAN Kohji

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• Kumagai Hidehiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Faculty Of Agriculture Kyoto University

• Yokoyama Tatsuo

  Institute For Fermentation Osaka

• Kumagai Hidehiko

  Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.

• Ohdan K

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Ohdan Kohji

  Department Of Food Science And Technology Facylty Of Agriculture Kyoto University

• Nunoura N

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University:(present Address)r

• Nunoura Naoki

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University Kitashirakawa

• Yano Toshihiro

  Department Of Food Science Ishikawa Agricultural College

• Kumagai H

  Department Of Agricultural Chemistry The University Of Tokyo

• Yano T

  Research Laboratory Of Higashimaru Shoyu Co. Ltd.

• Yamamoto Kenji

  Department Of Cardiovascular Surgery Kanagawa Cardiovascular And Respiratory Center

• KUMAGAI Hidehiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

• YANO Toshihiro

  Department of Food Science and Technology, Kyoto University

• YANO Toshihiro

  Department of Applied Chemistry, Nagoya Institute of Technology

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