Purification and Properties of an Enzyme Oxidizing Nitrite to Nitrate from Candida rugosa(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• A nitrite-oxidizing enzyme was isolated (1300-fold purification, 15% yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220,000) consisted of four identical subunits with molecular weight of 58,000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are desctibed.

• 社団法人日本農芸化学会の論文
• 1988-11-23

著者

• 酒井 謙二

  九大院生資環

• TAKANO Koji

  Department of Nephrology and Endocrinology, Faculty of Medicine, University of Tokyo

• Sakai Kenji

  Department of Plant Resources, Kyushu University

• TACHIKI TAKASHI

  Department of Food Science and Technology, Kyoto University

• TOCHIKURA TATSUROKURO

  Department of Food Science and Technology, Kyoto University

• Takano Koji

  Department Of Nephrology And Endocrinology University Of Tokyo Faculty Of Medicine

• Tochikura Tatsurokuro

  Faculty Of Home Economics Kobe Women's University

• Tochikura Tatsurokuro

  Department Of Agricultural Chemistry Kyoto University

• Tachiki Takashi

  Department Of Chemistry Ritsumeikan University

• Sakai Kenji

  Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University

• Tachiki Takashi

  Faculty Of Home Economics Kobe Women's University

• Tachiki Tkashi

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Tachiki Takashi

  Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit

• SAKAI Kenji

  Research Institute for Theoretical Physics Hiroshima University

• Takano Koji

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Takano Koji

  Department Of Chemistry Faculty Of Science Tokyo University Of Science

• Sakai Kenji

  Department Of Applied Chemistry Faculty Of Engineering Oita University

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