N-Terminal Region of Chitinase I of Bacillus circulans KA-304 Contained New Chitin-Biding Domain
スポンサーリンク
概要
- 論文の詳細を見る
Chitinase I (CHI1) of Bacillus circulans KA-304 forms protoplasts from Schizophyllum commune mycelia when the enzyme is combined with α-1,3-glucanase of B. circulans KA-304. CHI1 consists of an N-terminal unknown region and a C-terminal catalytic region classified into the glycoside hydrolase family-19 type. An N-terminal region-truncated mutant of CHI 1 (CatCHI1), which was expressed in Escherichia coli Rosetta-gami B (DE3), lost colloidal chitin- and powder chitin-binding activities. The colloidal chitin- and the powder chitin-hydrolyzing activities of CatCHI1 were lower than those of CHI1, and CatCHI1 was not effective in forming the protoplast. A fusion protein of the N-terminal region of CHI1 and green fluorescent protein (Nterm-GFP) was expressed in E. coli, and the fusion protein was adsorbed to colloidal chitin, powder chitin, and chitosan. Fluorescence microscopy analysis showed that Nterm-GFP bound to the S. commune cell-wall.
著者
-
Takagi Kazuyoshi
Department Of Surgery Kurume University School Of Medicine
-
TACHIKI TAKASHI
Department of Food Science and Technology, Kyoto University
-
YANO Shigekazu
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
-
SUYOTHA Wasana
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
-
HONDA Arata
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
-
RATTANAKIT-CHANDET Nopakarn
Department of Chemistry, Faculty of Science, Chiang Mai University
-
WAKAYAMA Mamoru
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
関連論文
- Exact Minimization of Free BDDs and Its Application to Pass-Transistor Logic Optimization (Special Section on VLSI Design and CAD Algorithms)
- Hardware Synthesis from C Programs with Estimation of Bit Length of Variables (Special Section on VLSI Design and CAD Algorithms)
- Purification and Characterization of Aromatic Amine Dehydrogenase from Alcaligenes xylosoxidans
- Electrochemical Control of Hydrogenase Action of Desulfovibrio vulgaris (Hildenborough)
- Quinone-Mediated Bioelectrochemical Reduction of NAD(P)+ Catalyzed by Flavoproteins
- N-Terminal Region of Chitinase I of Bacillus circulans KA-304 Contained New Chitin-Biding Domain
- Ammonia Assimilation by Glutamine Synthetase/Glutamate Syhthase System in Brevibacterium flavum
- Properties of Glutamate Synthase from Brevibacterium flavum
- Cloning and Expression of an α-1,3-Glucanase Gene from Bacillus circulans KA-304 : The Enzyme Participates in Protoplast Formation of Schizophyllum commune
- Cloning and Expression of a Bacillus circulans KA-304 Gene Encoding Chitinase I, Which Participates in Protoplast Formation of Schizophyllum commune
- Automated Passive-Transmission-Line Routing Tool for Single-Flux-Quantum Circuits Based on A* Algorithm
- A Method of Sequential Circuit Synthesis Using One-Hot Encoding for Single-Flux-Quantum Digital Circuits(Superconducting Electronics)
- Logic Synthesis Method for Dual-Rail RSFQ Digital Circuits Using Root-Shared Binary Decision Diagrams(VLSI Design Technology and CAD)
- Metabolism of N-Acetyl-D-amino Acid by Alcaligenes xylosoxydans subsp. xylosoxydans A-6
- Purification and Characterization of Nitrite-oxidizing Enzyme from Heterotrophic Bacillus badius I-73,with Special Concern to Catalase
- Analysis of the Microbial Community in an Activated Sludge Enriched with an Inorganic Nitrite Medium
- Role of Conserved Histidine Residues in D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6
- Purification and Characterization of _L-Aminoacylase from Pseudomonas maltophila B1
- L-Ornithine Decarboxylase from Hafnia alvei Has a Novel L-Ornithine Oxidase Activity
- Change in Nitrite Conversion Direction from Oxidation to Reduction in Heterotrophic Bacteria Depending on the Aeration Conditions
- Production and Localization of Enzymes on Soft Gel Cultivation(Microbiology & Fermentation Industry)
- Development of a Soft Gel Cultivation Method(Microbiology & Fermentation Industry)
- Production of Deoxyribonucleoside Triphosphates through Coupled Fermentation with Energy Transfer(Microbiology & Fermentation Industry)
- Synthesis of β-D-Fucosylglucose by β-D-Gliieosidase I of Bifidobacterium breve clb and Assimilation by Bifidobacteria
- Aortic Valve Replacement after Esophagectomy with Substernal Gastric Tube Reconstruction
- Infection management in a patient with a left ventricular assist device : a case report of long-term cefazolin sodium hydrate administration
- Dysfunction of an ATS valve in the aortic position : the first reported case caused by pannus formation
- Does Off-Pump Coronary Artery Bypass Grafting Really Preserve Renal Function?
- Floating-Point Euclidean Norm Computing Circuit
- Digit-Recurrence Algorithm for Computing Reciprocal Square-Root(Regular Section)
- A Hardware Algorithm for Integer Division Using the SD2 Representation(VLSI Design Technology and CAD)
- Automated Passive-Transmission-Line Routing Tool for Single-Flux-Quantum Circuits Based on A^* Algorithm
- A Clock Scheduling Algorithm for High-Throughput RSFQ Digital Circuits
- Cloning and Sequencing of a Gene Encoding D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and Expression of the Gene in Escherichia coli
- Purification and Some Properties of Glutaminase from Pseudomonas nitroreducens IFO 12694
- Characterization of α-Ketog1utarate-Resistant Sake Yeast Mutant with High Organic Acid Productivity
- Properties of Crystalline Glutamine Synthetase from Gluconobacter suboxydans Grown on Glutamate as a Nitrogen Source : Amino Acid Metabolism in Acetic Acid Bacteria (XI)
- Production of UDP-glucose by Torulopsis candida IFO 0768 : Studies on Microbial Metabolism of Sugar Nucleotides (VIII)
- Molecular Cloning and Characterization of γ-Glutamyltranspeptidase from Pseudomonas nitroreducens IFO12694
- Protoplast Formation from Schizophyllum commune by a Culture Filtrate of Bacillus circulans KA-304 Grown on a Cell-wall Preparation of S. commune as a Carbon Source
- γ-Glutamyl Transfer Reactions by Glutaminase from Pseudomonas nitroreducens IFO 12694 and Their Application for the Syntheses of Theanine and γ-Glutamylmethylamide
- Purification and Properties of an Enzyme Oxidizing Nitrite to Nitrate from Candida rugosa(Microbiology & Fermentation Industry)
- Conversion of Nitrite to Nitrate by Nitrite-resistant Yeasts(Microbiology & Fermentation Industry)
- Mortality of Bifidobacteria in Boiled Yogurt
- Increase in β-D-Glucoside-assimilating Ability of Bifidobacterium breve 203 Due to Acclimation to β-D-Glucoside(Microbiology & Fermentation Industry)
- Hydrolysis of α-D-Galactosyl Oligosaccharides in Soymilk by α-D-Galactosidase of Bifidobacterium breve 203(Microbiology & Fermentation Industry)
- Isolation and Characterization of Two β-D-Glucosidases from Bifidobacterium breve 203(Microbiology & Fermentation Industry)
- p-Nitrophenyl Glycoside-hydrolyzing Activities in Bifidobacteria and Characterization of β-D-Galactosidase of Bifidobacterium longum 401(Microbiology & Fermentation Industry)
- Analysis of Essential Amino Acid Residues for Catalytic Activity of Glutaminase from Micrococcus luteus K-3(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Nitrite Oxidation by Heterotrophic Bacteria under Various Nutritional and Aerobic Conditions
- Molecular Cloning and Determination of the Nucleotide Sequence of a Gene Encoding Salt-Tolerant Glutaminase from Micrococcus luteus K-3
- Production of D-Amino Acid Oxidase from Aspergillus sojae
- Chemical Modification of Histidine Residue of N-Acyl-D-Glutamate Amidohydrolase from Pseudomonas sp. 5f-1
- Cloning, Expression, and Nucleotide Sequence of the N-Acyl-_D-Aspartate Amidohydrolase Gene from Alcaligenes xylosoxydans subsp. sylosoxydans A-6
- Metal-characterization of N-Acyl-D-glutamate Amidohydrolase from Pseudomonas sp. Strain 5f-1
- Primary Structure of N-Acyl- D-Glutamate Amidohydrolase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6^1
- Isolation, Enzyme Production and Characterization of D-Aspartate Oxidase from Fusarium sacchari var. elongatum Y-105
- Isolation and Characterization of Salt-Tolerant Glutaminases from Marine Micrococcus luteus K-3
- Production, Purification, and Characterization of D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6
- Cloning and Expression of Methylovorus mays No. 9 Gene Encoding γ-Glutamylmethylamide Synthetase: An Enzyme Usable in Theanine Formation by Coupling with the Alcoholic Fermentation System of Baker’s Yeast
- Theanine Production by Coupled Fermentation with Energy Transfer Using γ-Glutamylmethylamide Synthetase of Methylovorus mays No. 9
- Purification of Aspergillus sp. S1-13 Chitinases and Their Role in Saccharification of Chitin in Mash of Solid-State Culture with Shellfish Waste(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Characterization of Theanine-Forming Enzyme from Methylovorus mays No. 9 in Respect to Utilization of Theanine Production
- Cloning and Expression of Pseudomonas taetrolens Y-30 Gene Encoding Glutamine Synthetase : An Enzyme Available for Theanine Production by Coupled Fermentation with Energy Transfer
- Theanine Production by Coupled Fermentation with Energy Transfer Employing Pseudomonas taetrolens Y-30 Glutamine Synthetase and Baker's Yeast Cells
- Purification and Characterization of Glutamine Synthetase of Pseudomonas taetrolens Y-30 : An Enzyme Usable for Production of Theanine by Coupling with the Alcoholic Fermentation System of Baker's Yeast
- A Chitinase Indispensable for Formation of Protoplast of Schizophyllum commune in Basidiomycete-lytic Enzyme Preparation Produced by Bacillus circulans KA-304
- Occurrence of a Specific Protein in Basidiomycete-lytic Enzyme Preparation Produced by Bacillus circulans KA-304 Inductively with a Cell-wall Preparation of Schizophyllum commune(Microbiology & Fermentation Technology)
- Saccharification of Chitin Using Solid-State Culture of Aspergillus sp.S1-13 with Shellfish Waste as a Substrate
- Utilization of Shrimp Shellfish Waste as a Substrate for Solid-State Cultivation of Aspergillus sp. S1-13 : Evaluation of a Culture Based on Chitinase Formation Which Is Necessary for Chitin-Assimilation
- Glutamate Formation by a New In Virto Enzyme System Consisting of Purified Clutamine Synthetase and Glutamate Synthase
- Purification and Some Properties of Glutamate Synthase from Gluconobacter suboxydans grown on Glutamate as a Nitrogen Source
- Purification and Some Properties of Glutamine Synthetases from Bifidobacteria(Microbiology & Fermentation Industry)
- Distribution and Some Properties of Glutamine Synthetase and Glutamate Dehydrogenase in Bifidobacteria(Microbiology & Fermentation Industry)
- Molecular Cloning and Characterization of γ-Glutamyltranspeptidase from Pseudomonas nitroreducens IFO12694
- Role of Chitin Binding Domain of Chitinase A of Streptomyces cyaneus SP-27 in Protoplast Formation from Schizophyllum commune
- Cloning and Expression of Chitinase A Gene from Streptomyces cyaneus SP-27 : The Enzyme Participates in Protoplast Formation of Schizophyllum commune
- Purification and Characterization of Chitinase A of Streptomyces cyaneus SP-27 : An Enzyme Participates in Protoplast Formation from Schizophyllum commune Mycelia
- N-Terminal Region of Chitinase I of Bacillus circulans KA-304 Contained New Chitin-Biding Domain
- Repeated Batch Production of Theanine by Coupled Fermentation with Energy Transfer Using Membrane-Enclosed γ-Glutamylmethylamide Synthetase and Dried Yeast Cells
- Theanine Production by Coupled Fermentation with Energy Transfer Using γ-Glutamylmethylamide Synthetase of Methylovorus mays No. 9
- Cloning and Expression of Methylovorus mays No. 9 Gene Encoding γ-Glutamylmethylamide Synthetase : An Enzyme Usable in Theanine Formation by Coupling with the Alcoholic Fermentation System of Baker's Yeast
- Characterization of Theanine-Forming Enzyme from Methylovorus mays No. 9 in Respect to Utilization of Theanine Production
- Microwave-induced zero-crossing steps observed in BSCCO intrinsic Josephson junctions
- Millimeter-wave response mechanism of slot antenna coupled YBCO microbridge
- Properties of Brevibacterium flavum Glutamine Synthetase in an "in vivo-like" System
- Production of ATP from Adenine by a Combination of Bacterial and Baker's Yeast Cells
- Fermentative Production of Inosine 5'-Phosphate from Adenosine by Baker's Yeast
- Gum chewing enhances early recovery of bowel function following transperitoneal abdominal aortic surgery
- Occurrence of Glutamate Synthase in Brevibacterium flavum
- Domain Structure and Function of α-1,3-Glucanase from Bacillus circulans KA-304, an Enzyme Essential for Degrading Basidiomycete Cell Walls