Cloning, Expression, and Nucleotide Sequence of the N-Acyl-_D-Aspartate Amidohydrolase Gene from Alcaligenes xylosoxydans subsp. sylosoxydans A-6

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概要

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• The gene (termed daa) encoding N-acyl-D-aspartate (D-Asp) amidohydrolase (D-AAsae) from the Alcaligenes xylosoxydans subsp. xylosoxydans (Alcaligenes A-6) was cloned in Esherichia coli (E. coli) JM109. The daa gene consists of 1,494 nucleotides and encodes 498 amino acid residues. The molecular weight of D-AAase was caluculated to be 53,581. The N-terminal amino acid sequence (NH_2-TDRSTLDDAP-) predicted by the nucleotide sequence matched exactly thse of the purified D-AAase form both Alcaligenes A-6 and cloned E. coli, with the exception of the removal of the N-terminal methionine processed after translation. A comparison of the amino acid sequence of D-AAase with that of D-aminoanylase from Alcaligenes A-6 showed high overall homology (56%). D-AAase from Alcaligenes A-6 showed 25〜29% homology with Bacillus with stearothermophilus, porcine, and human _L-aminoacylases. The daa was highly expressed in E. coli, and the recombinant enzyme was purified to homogeneity with 17.8% yield.

• 社団法人日本生物工学会の論文
• 1995-10-25

著者

• 酒井 謙二

  九大院生資環

• Sakai Kenji

  Department of Plant Resources, Kyushu University

• WAKAYAMA Mamoru

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• Miura Y

  Department Of Applied Biological Science Tokyo Noko University

• Miura Yutaka

  Applied Bioresearch Center Kirin Brewry Co. Ltd.

• Miura Yutaka

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• MORIGUCHI Mitsuaki

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• TAU Yuko

  Department of Applied Chemistry, Faculty of Engineering, Oita University

• TAKENAKA YASUHIRO

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• Miyamoto Yoshiro

  Department Of Biochemical Engineering Faculty Of Computer Science And System Engineering Kyushu Inst

• Tau Yuko

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Wakayama Mamoru

  Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University

• Moriguchi Mitsuaki

  Department Of Food And Bioscience Beppu University

• Moriguchi Mitsuaki

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• WATANABE Eiki

  Department of Electrical Engineering, Tokyo Metropolitan University

• Takenaka Y

  Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.

• Watanabe Eiki

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Mori Masatsugu

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Sakai Kenji

  Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University

• SAKAI Kenji

  Research Institute for Theoretical Physics Hiroshima University

• Takenaka Yasuhiro

  Department Of Advanced Bioscience Kinki University

• Takenaka Yasuhiro

  Department Of Applied Chemistiry Faculty Of Engineering Oita University

• Miura Y

  Tokyo Noko Univ. Tokyo Jpn

• Miura Yutaka

  Dep. Of Applied Biological Sci. Tokyo Noko Univ.

• Sakai Kenji

  Department Of Drug Metabolism And Pharmacokinetics Nippon Boehringer Ingelheim Co. Ltd.

• Miyamoto Yoko

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Sakai Kenji

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Wakayama Mamoru

  Department of Applied Chemistry, Faculty of Engineering, Oita University

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