Cloning and Expression of Chitinase A Gene from Streptomyces cyaneus SP-27 : The Enzyme Participates in Protoplast Formation of Schizophyllum commune

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概要

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• Chitinase A of Streptomyces cyaneus SP-27 or chitinase I of Bacillus circulans KA-304 showed the protoplast-forming activity when combined with α-1,3-glucanase of B. circulans KA-304.The gene of chitinase A was cloned. It consisted of 903 nucleotides encoding 301 amino acid residues, including a putative signal peptide (35 amino acid residues). The deduced N-terminal moiety of chitinase A showed sequence homology with the chitin-binding domain of chitinase F from Streptomyces coelicolor and chitinase 30 from Streptomyces olivaceoviridisis. The C-terminal moiety also showed high sequence similarity to the catalytic domain of several Streptomyces family 19 chitinases as well as that of chitinase I of B. circulans KA-304.Recombinant chitinase A was expressed in Escherichia coli Rosetta-gami B (DE 3). The properties of the recombinant enzyme were almost the same as those of chitinase A purified from a culture filtrate of S. cyaneus SP-27. The recombinant enzyme was superior to B. circulans KA-304 chitinase I not only in respect to protoplast forming activity in a mixture containing α-1,3-glucanase, but also to antifungal activity and powder chitin-hydrolyzing activity.

• 社団法人 日本農芸化学会の論文
• 2008-07-23

著者

• TACHIKI TAKASHI

  Department of Food Science and Technology, Kyoto University

• YANO Shigekazu

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• HONDA Arata

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• WAKAYAMA Mamoru

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• Wakayama Mamoru

  Dep. Of Biotechnology Fac. Of Life Sciences Ritsumeikan Univ.

• Wakayama Mamoru

  Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University

• Rattanakit Nopakarn

  Department of Chemistry, Faculty of Science, Chiang Mai University

• Tachiki T

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Tachiki Takashi

  Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit

• Yano Shigekazu

  Department Of Biotechnology College Of Life Sciences Ritsumeikan University

• Rattanakit Nopakarn

  Department Of Chemistry Faculty Of Science Chiang Mai University

• NODA Yuta

  Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University

• PLIKOMOL Abhinya

  Department of Biology, Faculty of Science, Chiang Mai University

• Yano Shuntaro

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Honda Arata

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Wakayama Mamoru

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Noda Yuta

  Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University

• Plikomol Abhinya

  Department Of Biology Faculty Of Science Chiang Mai University

• YANO Shigekazu

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• Wakayama Mamoru

  Department of Applied Chemistry, Faculty of Engineering, Oita University

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