Purification and Properties of Acinetobacter sp. Endo-β-N-Acetylglucosaminidase Acting on Complex Olgosaccharides of Glycoproteins
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概要
- 論文の詳細を見る
A novel endo-β-N-acetylglucosaminidase capable of acting on complex type sugar chains of glycoproteins was found in the culture broth of a bacterium which was isolated from soil and identified as Acinetobacter sp. The enzyme was purified to homogeneity on polyacrylamide gel electrophoresis by successive purification procedures involving ammonium sulfate fractionation and chromatographies on DEAE-cellulose, hydroxylapatite and Sephadex G-150. Its molecular weigh was about 35,000 on gel filtration. The optimum pH was 3.0-3.5,and the enzyme was stable in the pH range from 6-8. The enzyme had high activity on dansyl ovalbumin glycopeptide, and also could hydrolyze dansyl asialotransferrin glycopeptide and dansyl transferrin glycopeptide containing complex type sugar chains. The K_m value for dansyl asialotransferring glycopeptide as the substrate of enzyme assay was 0.68mM. THe enzyme could release complex type sugar chains from intact asialotransferrin without the addition of any detergent.
- 社団法人日本生物工学会の論文
- 1991-04-25
著者
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YAMAMOTO Kenji
Department of Medical Ecology and Informatics, Research Institute, International Medical Center of J
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TSUCHIDA TAKAMASA
Department of Medical Bioengineering and Sports Medicine, Hokkaido University School of Medicine
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Yamamoto K
Graduate School Of Biostudies Kyoto University
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TOCHIKURA TATSUROKURO
Department of Food Science and Technology, Kyoto University
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KADOWAKI Setsu
Kacho College
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FUJISAKI Masatoki
Department of Food Science and Technology, Kyoto University
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Tsuchida Takamasa
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Tochikura Tatsurokuro
Faculty Of Home Economics Kobe Women's University
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Tochikura Tatsurokuro
Department Of Food Science And Technology Kyoto Unicersity
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Tochikura Tatsurokuro
Department Of Agricultural Chemistry Kyoto University
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Kadowaki S
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University Kacho Junior Colle
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Tachiki Takashi
Department Of Chemistry Ritsumeikan University
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Tachiki T
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Tachiki Takashi
Faculty Of Home Economics Kobe Women's University
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Tachiki Tkashi
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Fujisaki Masatoki
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Yamamoto Kenji
Department Of Cardiovascular Surgery Kanagawa Cardiovascular And Respiratory Center
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Yokozeki Kenzo
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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