Suppression of Gingival Inflammation Induced by Porphyromonas gingivalis in Rats by Leupeptic

スポンサーリンク

概要

• 論文の詳細を見る

• In this study, we developed a procedure to produce gingivitis in rats by inoculation of Porphyromonas gingivalis and studied the contribution of the bacterial cysteine proteinases, Arg-gingipain (Rgp) and Lys-gingipain (Kgp), to the pathology in the gingiva. To adhere the bacterium to periodontal tissues, a cotton thread was inserted between the first and second molar of right maxillary sites of rats. Rats in group A were administered with vehicle alone after bacterial (strain W83) inoculation. In group B, the bacteria were inoculated in combination with leupeptin, a potent inhibitor of Rgp and Kgp, and then leupeptin alone was administered the week after. Rats in group C were administered leupeptin for 6 weeks after bacteria inoculation. All left maxillary gingiva in three groups showed no inflammatory changes. Right maxillary gingiva of group A showed most of the clinical landmarks of gingivitis. Leupeptin exhibited only a little inhibitory effect on this gingivitis in group B, whereas it had a strong inhibitory effect on the inflammation in group C. These results suggest that P. gingivalis-induced gingivitis is attributable to Rgp and Kgp and that leupeptin is more effective in the late phase than the early stage of gingivitis.

• 社団法人 日本薬理学会の論文
• 2001-01-01

著者

• YAMAMOTO Kenji

  Department of Medical Ecology and Informatics, Research Institute, International Medical Center of J

• Baba A

  Laboratory Of Molecular Neuropharmacology Graduate School Of Pharmaceutical Sciences Osaka Universit

• Irimura Kenji

  Drug Safety Research Laboratory, Taiho Pharmaceutical Co., Ltd.

• ABE Naoko

  Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century C

• Abe N

  Department Of Pharmacology Faculty Of Dentistry Kyushu University

• Abe Naoko

  Department Of Bioengineering Faculty Of Engineering

• Irimura Kenji

  Drug Safety Research Labolatory Taiho Pharmaceutical Co. Ltd.

• Irimura Kenji

  Drug Safety Research Laboratory Tokushima Research Center Taiho Pharmaceutical Co. Ltd.

• Baba A

  Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University

• Yamamoto Kenji

  Proteolysis Research Laboratory Graduate School Of Pharmaceutical Sciences Kyushu University

• Abe N

  Department Of Bioengineering Faculty Of Engineering

• MIYAKE Yoichiro

  Department of Thoracic and Cardiovascular Surgery, Mie University School of Medicine

• BABA Atsuyo

  Department of Phyrmacology and Microbiology,Kyushu University Faculty of Dentisty

• Sasaki T

  Drug Discovery Pharmaceutical Research Center Meiji Seika Kaisha Ltd.

• Yamamoto K

  Department Of Pharmacology Graduate School Of Dental Science Kyushu University

• Kato Yuzo

  Division Of Oral Molecular Pharmacology Department Of Developmental And Reconstructive Medicine Naga

• Baba Atsuyo

  Department Of Pharmacology Graduate School Of Dental Science Kyushu University

• SASAKI Toru

  Pharmaceutical Research Center, Meiji Seika, Ltd.

• Miyake Y

  Department Of Biological Science Graduate School Of Science Osaka University

• Miyake Yoichiro

  Department Of Microbiology Institute Of Health Biosciences The University Of Tokushima Graduate Scho

• Miyake Yoichiro

  Department Of Pharmacology Faculty Of Dentistry Kyushu University

• KITANO Sizuo

  Pharmacology Research Laboratory, Tokushima Research Center, Taiho Pharmaceutical Co., Ltd.

• KATSUNUMA Nobuhiko

  Institute of Health Sciences, Tokushima Bunri University

• Kitano Sizuo

  Pharmacology Research Laboratory Tokushima Research Center Taiho Pharmaceutical Co. Ltd.

• Sasaki Toru

  Pharmaceutical Research Center Meiji Seika Kaisha Ltd.

• Katsunuma Nobuhiko

  Institute Of Health Sciences Tokushima Bunri University

• Yamamoto K

  Department Of Allergy And Rheumatology Faculty Of Medicine University Of Tokyo

• Yamamoto Kenji

  Department Of Cardiovascular Surgery Kanagawa Cardiovascular And Respiratory Center

• Sasaki Toru

  Pharmacology Research Laboratory Tokushima Research Center Taiho Pharmaceutical Co. Ltd.

• Irimura Kenji

  Drug Safety Laboratory Taiho Pharmaceutical Co. Ltd.

• Katunuma N

  Institute of Health Sciences, Tokushima Bunri University

関連論文

• Novel Surface Processing with Sulfonic Acid for Quantum Dot and Its Characteristics
• On the Cyto-Toxicity Caused by Quantum Dots
• Altered Expression of γ-Glutamylcysteine Synthetase, Metallothionein and Topoisomerase I or II during Acquisition of Drug Resistance to Cisplatin in Human Ovarian Cancer Cells
• No evidence for significant association between GABA receptor genes in chromosome 15q11-q13 and autism in a Japanese population
• Tachykinin 1 (TAC1) gene SNPs and haplotypes with autism : A case-control study
• Serotonin transporter gene promoter polymorphism and autism : A family-based genetic association study in Japanese population
• No association of FOXP2 and PTPRZ1 on 7q31 with autism from the Japanese population
• Cyclic AMP Phosphodiesterase 4 Isoenzyme Inhibitory Activity of (R)- and (S)-Isomer of 7-Methyl- or 8-Alkyl-4, 5, 7, 8-tetrahydroimidazo [2,1-i] -purin-5-one (Highlihted paper slected by Editor-in-chief)(Medicinal Chemistry)
• Synthesis and Cyclic AMP Phosphodiesterase 4 Isoenzyme Inhibitory Activity of Heterocycle Condensed Purines
• Phosphodiesterase 4 Isoenzyme Inhibitory Activity of 3-Phenylxanthines and 4-Phenyl[i]condensed-purines
• Condensed-Purines Syntheses of Tetrahydro-1,4-diazepino[1,2,3-gh]purin-2-one and Hexahydro-1,4-diazocino[1,2,3-gh]purin-2-one
• Structural Analysis of Disaccharides Synthesized by β-D-Glucosidase of Bifidobacterium breve clb and Their Assimilation by Bifidobacteria
• Transglycosylation Activity of β-N-Acetylhexosaminidase from Penixillium pxalicum and Its Application to Synthesis of a Drug Carrier
• Analysis of Low Temperature Inducible Mechanism of γ-Glutamyltranspeptidase of Escherichia coli K-12
• Cloning and Nucleotide Sequence of the β-D-Glucosidase Gene from Bifidobacterium breve clb, and Expression of β-D-Glucosidase Activity in Escherichia coli
• Mapping, Cloning, and DNA Sequencing of pepB Which Encodes Peptidase B of Escherichia coli K-12
• Screening for Bacterial Strains Producing Lactate Oxidase
• Transcriptional Regulation of Tyrosine Phenol-Lyase Gene of Erwinia herbicola AJ2985
• Subunit Association of γ-Glutamyltranspeptidase of Escherichia coli K-12^1
• Formation of Lyso-glycosphingolipids by Streptomyces sp.
• Effect of Site-Directed Mutations on Processing and Activity of γ-Glutamyltranspeptidase of Escherichia coli K-12
• OJ-255 A Possible Role of Periodontitis in Acute Myocardial Infarction(Acute myocardial infarction, clinical (pathophysiology)-2 (IHD) OJ43,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• Characterization of New Fluorogenic Substrates for the Rapid and Sensitive Assay of Cathepsin E and Cathepsin D^1
• Deglycosylated Glucoamylase from Rhizopus niveus is Precipitated by Flavobacterium sp. Endo-β-N-acetylglucosaminidase(Microbiology & Fermentation Industry)
• Angiostatin : An Endogenous Inhibitor of Angiogenesis and Tumor Growth
• Thallium-201 brain SPECT to diagnose aggressiveness of meningiomas
• Differential diagnosis in patients with ring-like thallium-201 uptake in brain SPECT
• Biofeedback Training for Bronchial Asthma (Part I)
• Unique Production of a Disaccharide, Galβ1→3GalNAc, by Endo-α-N-Acetylgalactosaminidase of Alcaligenes sp.
• Preservation of Raw Fish and Meat(Food & Nutrition)
• Peptide Production from Protein without Salt(Microbiology & Fermentation Industry)
• Induction and Efficient Purification of Endo-α-N-acetylgalactosaminidase from Alcaligenes sp.(Microbiology & Fermentation Industry)
• Isolation and Characterization of a Blood Group A Substancedegrading α-N-Acetylgalactosaminidase from an Acremonium sp.
• A Novel End-β-N-acetylglucosaminidase Acting on Complex Oligosaccharides of Glycoproteins in a Fungus (Microbiological Fermentation Industry)
• Effect of Yeast Extract on Endo-β-N-acetylglucosaminidase Production by a Flavobacterium sp.(Microbiology & Fermentation Industry)
• Purification, Characterization, and Sequencing of Two Cysteine Proteinase Inhibitors, Sca and Scb, from Sunflower (Helianthus annuus) Seeds
• Characterization of Phagosomal Subpopulations along Endocytic Routes in Osteoclasts and Macrophages
• An Effect of Appreciating Music on Emotion : Follow Up of the Profile of Mood States Score Before and After Appreciating Music
• The treatment of neuroleptic malignant syndrome using dantrolene sodium
• Biofeedback Training for Bronchial Asthma (Part II)
• On the Trend of Biofeedback Studies as seen in CIM
• An Involvement of Granulocyte Medullasin in Phenytoin-Induced Gingival Overgrowth in Rats
• Effects of localized lesions in the lateral suprasylvian cortex on convergence eye movement in cats
• The Regulation of Bone Resorption in Tooth Formation and Eruption Processes in Mouse Alveolar Crest Devoid of Cathepsin K
• Gingipains Inactivate a Cell Surface Ligand on Porphyromonas gingivalis That Induces TLR2- and TLR4-Independent Signaling
• Rapid Degradation of Cathepsin E in Pre-Golgi Compartments after Tunicamycin Treatment
• Simultaneous Multicolor Detection System of the Single-Molecular Microbial Antigen with Total Internal Reflection Fluorescence Microscopy
• Deglycosylation of Hen Ovalbumin in Native Form by Endo-β-N-Acetylglucosaminidase(Food & Nutrition)
• Electronic and Structural Requirements for Metabolic Activation of Butylated Hydroxytoluene Analogs to Their Quinone Methides, Intermediates Responsible for Lung Toxicity in Mice
• Purification and Characterization of a Protease-Resistant Cellulase from Aspergillus niger
• Primary Structure of an O-Linked Sugar Chain Derived from Glucose Oxidase of Aspergillus niger(Biological Chemistry)
• The Release of Oligosaccharides from Glycoproteins by Endo-β-N-Acetylglucosaminidase of Flavobacterium sp.
• Elucidation of the Role of Sugar Chains in Glycosylated-enzymes Using Endo-β-N-acetylglu-cosaminidase from Flavo-bacterium sp.
• Cathepsin E-Deficient Mice Show Increased Susceptibility to Bacterial Infection Associated with the Decreased Expression of Multiple Cell Surface Toll-Like Receptors
• Association of Cathepsin E Deficiency with Development of Atopic Dermatitis
• Porphyromonas gingivalis Proteinases as Virulence Determinants in Progressin of Periodonatal Diseases
• Biochemical and Functional Properties of Lysine-Specific Cysteine Proteinase(Lys-Gingipain)as a Virulence Factor of Porphyromonas gingivalis in Periodontal Disease^1
• Cloning and Sequencing of the Gene Encolding a Novel Lysine-Specific Cysteine Proteinase (Lys-Gingipain)in Porphyromonas gingivalis: Structural Relationship with the Arginine-Specific Cysteine Proteinase(Arg-Gingipain)
• Biochemical Properties of the Monomeric Mutant of Human Cathepsin E Expressed in Chinese Hamster Ovary Cells: Comparison with Dimeric Forms of the Natural and Recombinant Cathepsin E
• Structure-Activity Relationships of Alkylxanthine Inhibitors of Phosphodiesterase IV Isoenzyme
• Novel Specificities of Mucor hiemalis Endo-β-N-acetylglucosaminidase Acting Complex Asparagine-Linked Oligosaccharides
• Up-regulation, Enhanced Maturation, and Secretion of Cathepsin E in Mouse Macrophages Treated with Interferon-γ or Lipopolysaccharide
• The Role of the Cathepsin E Propeptide in Correct Folding, Maturation and Sorting to the Endosome
• Mutational Analysis of Residues in Two Consensus Motifs in the Active Sites of Cathepsin E
• Design and Synthesis of Sensitive Fluorogenic Substrates Specific for Lys-Gingipain^1
• Multiple Cavernous Hemangiomas of the Skull Associated With Hepatic Lesions : Case Report
• The Effect of Magnesium Ion on Yeast UDP-N-acetylglucosamine Pyrophosphorylase and Its Activation by Dithiothreitol
• A Novel Type of Two-Component Regulatory System Affecting Gingipains in Porphyromonas gingivalis
• Selective Proteolysis of Apolipoprotein B-100 by Arg-Gingipain Mediates Atherosclerosis Progression Accelerated by Bacterial Exposure
• Fragile X carrier screening and FMR1 allele distribution in the Japanese population
• Activation of Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae by Various Sugars
• PROJECTIVE TRANSFORMATION TO ALIGN MULTI-CAMERA IMAGES FOR EFFICIENT FTV COMPRESSION(International Workshop on Advanced Image Technology 2005)
• Quantum Dots Targeted to the Assigned Organelle in Living Cells
• Novel non-apoptotic morphological changes in neurons of the mouse hippocampus following transient hypoxic-ischemia
• Application of the relaxation technique in general hospital psychiatry
• Effects of Size of Carbohydrate Chain on Protease Digestion of Aspergillus niger Endo-β-I,4-glucanase
• Delivery outcomes of gasless laparoscopic ovarian cystectomy during pregnancy(Session14 Others3)
• Purification and Properties of Acinetobacter sp. Endo-β-N-Acetylglucosaminidase Acting on Complex Olgosaccharides of Glycoproteins
• Purification and Characterization of a Novel Fungal Endo-β-N-acetylglucosaminidase Acting on Complex Oligosaccharides of Glycoproteins(Microbiology & Fermentation Industry)
• Purification and Characterization of Endo-α-N-acetylgalactosaminidase from Alcaligenes sp.(Microbilolgy & Fermentation Industry)
• Isolation of Endo-α-N-acetylgalactos-aminidase from an Aerobic Bacterium(Microbiology & Fermentation Industry)
• Surgical results of low-dose systemic heparinization 1mg/kg, 100IU/kg) in thoracic aortic operation combined with a heparin-coated cardiopulmonary bypass circuit : Is low-dose heparinization safe during deep hypothermic circulatory arrest?
• Cooperative Action of Arg- and Lys-gingipains in Disruption of Fibronectin-Integrin Interactions in Human Gingival Fibroblasts
• Low Temperature Vapor Phase Epitaxy of Undoped ZnSe Films on (100) GaAs Using Metallic Zn and Se
• Suppression of Gingival Inflammation Induced by Porphyromonas gingivalis in Rats by Leupeptic
• New Ligands with a Wide Bite Angle. Efficient Catalytic Activity in the Rh(I)-Catalyzed Hydroformylation of Olefins
• Purification and Characterization of β-D-Glucosidase (β-D-Fucosidase) from Bifidobacterium breve clb Acclimated to Cellobiose
• Quantitative Analysis of the Growth of Bacillus circulans on a Slant Agar Medium
• Photodegradative Surfactants : Photolysis of p-Dodecylbenzyltrimethylammonium Bromide in Aqueous Solution
• Monitoring the Transcription and Transport Process of RNA Using Optical Imaging Techniques: A Brief Review
• LECT2 intron/exson structure : potential strategies for bio-imaging
• Collective Fluorescence Oscillation in a Water Dispersion of Colloidal Quantum Dots
• Approaches to the treatment of somatoform disorders in different settings : Liaison psychiatry
• Photodegradative Surfactants : p-Dodecylbenzyltrimethylammonium Bromide as a Photodegradative Emulsifier for Microemulsion Polymerization
• Condensation Reactions of Amino Acids under Hydrothermal Conditions with Adiabatic Expansion Cooling
• High-Density Diagnostics for Laser-Imploded Plasmas
• A Quantum Scattering Approach to the Level Shift of Spectral Lines in Plasmas
• On the Complex Level Shift of Spectral Lines in Plasmas
• Lanthanide Ion as a Catalyst for Internucleotide Bond Formation
• Quantum Scattering Theory of the Complex Level Shift of Spectral Lines in Plasmas

もっと見る 閉じる

スポンサーリンク