Effects of Size of Carbohydrate Chain on Protease Digestion of Aspergillus niger Endo-β-I,4-glucanase
スポンサーリンク
概要
- 論文の詳細を見る
Three different carbohydrate-depleted enzymes were prepared from an endo-β-1, 4-glucanase of Aspergillus niger IFO31125 by treatment with endo-β-N-acetylglucosaminidase or α-mannosidase. They were purified by Concanavalin A-Sepharose affinity and DEAE ion-exchange column chromatographies. The molecular sizes of these enzymes had been decreased from 40 kDa containing 9.0% carbohydrate to 39, 38, and 37 kDa with carbohydrate at 4.5, 1.3, and 0.8% (wt/wt), respectively. The native and these carbohydrate-depleted enzymes were compared in their enzymatic properties, and it was found that they were identical in their catalytic activities and both thermal and pH stabilities. However, the 37-kDa enzyme was more susceptible to proteolysis by Savinase, proteinase K, and Pronase E. On the other hand, the specific protease trypsin showed no such effect on activity of all enzymes. These results suggested that the core structure of the asparagine-linked sugar chain, which consisted of three monosaccharide residues, contributed to the high stability of the endo-β-1, 4-glucanase against protease digestion.
- 社団法人日本農芸化学会の論文
- 1995-06-23
著者
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YAMAMOTO Kenji
Department of Medical Ecology and Informatics, Research Institute, International Medical Center of J
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Kumagai H
Kyoto Univ. Kyoto Jpn
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Yamamoto K
Graduate School Of Biostudies Kyoto University
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KUMAGAI HIDEHIKO
Department of Food Science and Technology, Kyoto University
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AKIBA SHUNICHI
Biological Science Laboratories, Kao Corporation
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Akiba Shunichi
Biological Science Laboratories Kao Corporation
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Kumagai H
Kyoto Univ.
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Kumagai H
Nihon Univ. Fujisawa‐shi Jpn
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Kumagai Hidehiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Kumagai Hidehiko
Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.
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Yamamoto Kenji
Department Of Cardiovascular Surgery Kanagawa Cardiovascular And Respiratory Center
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Yokozeki Kenzo
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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KUMAGAI Hidehiko
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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