Activation of Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae by Various Sugars
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概要
- 論文の詳細を見る
Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was activated by the addition of glucose, mannose, N-acetylglucosamine, and β-allose. While the enzyme did not appear to the significantly affected by the addition of galactose or N-acetylgalactosamine. These results indicate that the C-4 and C-6 positions of the monosaccharide are the most important for enzyme activation. Moreover, the enxyme was activated by the addition of disaccharides such as cellobiose, gentiobiose, and di-N-acetylchitobiose, but not by polysaccharides such as starch and yeast mannan. In the presence of N-acetylglucosamine, the enzyme activation occurred well over pH40. and the K_m value of the enzyme for (Man)_6(GlcNAc)_2-Asn-dansyl changes from 1.2mM to 3.2mM.
- 社団法人日本生物工学会の論文
- 1991-04-25
著者
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YAMAMOTO Kenji
Department of Medical Ecology and Informatics, Research Institute, International Medical Center of J
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TAKEGAWA Kaoru
Department of Life Science, Faculty of Agriculture, Kagawa University
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Takegawa K
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Takegawa Kaoru
Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.
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Takegawa K
Kagawa Univ. Kagawa Jpn
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Yamamoto K
Graduate School Of Biostudies Kyoto University
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IWAHARA SHOJIRO
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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Iwahara S
Kagawa Univ. Kagawa Jpn
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Tochikura Tatsurokuro
Faculty Of Home Economics Kobe Women's University
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Tochikura Tatsurokuro
Department Of Food Science And Technology Kyoto Unicersity
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Tochikura Tatsurokuro
Kobe Women's University
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Takegawa Kaoru
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios
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Tachiki Takashi
Department Of Chemistry Ritsumeikan University
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Tochikura T
Kobe Women's University
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NAKOSHI MASANAO
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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TACHIKURA TATSUROKURO
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Tachiki T
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Iwahara Shojiro
Department Of Agricultural Chemistry Faculty Of Agriculture Kagawa University
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Tachiki Takashi
Faculty Of Home Economics Kobe Women's University
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Tachiki Tkashi
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Nakoshi Masanao
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Yamamoto Kenji
Department Of Cardiovascular Surgery Kanagawa Cardiovascular And Respiratory Center
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Yokozeki Kenzo
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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