Transglycosylation Activity of β-N-Acetylhexosaminidase from Penixillium pxalicum and Its Application to Synthesis of a Drug Carrier

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概要

• 論文の詳細を見る

• Penicillium oxalicum β-N-acetylhexosaminidase was found to have transglycosylation activity in addition to hydrolytic acitivity. The enzyme effectively transferred N-acetylglucosamine to various alcohols in the presence of N-acetylchitooligomers as donors. The enzume also transferred N-acetylglucosamine to diol-derivatives. Using this transglycosylation activity, a drug carrier, 6-benzyloxyhexyl-β-N-acetylglucosaminidem, was synthesized by incubating the enzyme with 6-benzyloxyhexan-1-ol and N-acetylchitotriose. The product was isolated by sliica gel column chromatography and was analyzed by mass-spectrometry and ^1H-NMR.

• 社団法人日本生物工学会の論文
• 1997-04-25

著者

• YAMAMOTO Kenji

  Department of Medical Ecology and Informatics, Research Institute, International Medical Center of J

• WATANABE Jun

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Kumagai H

  Kyoto Univ. Kyoto Jpn

• Kumagai Hidehiko

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• KUMAGAI HIDEHIKO

  Department of Food Science and Technology, Kyoto University

• Saskiawan Iwan

  Graduate School Of Biostudies Kyoto University

• KADOWAKI SETSU

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kacho Junior Co

• SASKIAWAN IWAN

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kacho Junior Co

• BUNNO MASAYASU

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kacho Junior Co

• ICHIHARA YOSHIHIRO

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kacho Junior Co

• Kumagai Hidehiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.

• Kumagai Hidehiko

  Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University

• Kadowaki S

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University Kacho Junior Colle

• Bunno Masayasu

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University Kacho Junior Colle

• Kumagai H

  Department Of Agricultural Chemistry The University Of Tokyo

• Watanabe Jun

  Department Of Anatomy & Cell Science Kansai Medical University

• Kumagai Hidehiko

  Graduate School Of Biostudies Kyoto University

• Yamamoto Kenji

  Department Of Cardiovascular Surgery Kanagawa Cardiovascular And Respiratory Center

• Ichihara Yoshihiro

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University Kacho Junior Colle

• Watanabe Jun

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University Kacho Junior Colle

• KUMAGAI Hidehiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

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