Ammonia Assimilation by Glutamine Synthetase/Glutamate Syhthase System in Brevibacterium flavum
スポンサーリンク
概要
- 論文の詳細を見る
The formation of glutamine synthetase (GS) and glutamate synthase (GOGAT) in Brevibacterium flavum (ATCC 14607) was repressed by ammonia in the culture medium. Glutamate dehydrogenase (GDH)-defective mutants could grow in ammonia medium but glutamate dehydrogenase (GDH)-glutamine synthetase (GS) or glutamate dehydrogenase (GDH)-glutamate synthase (GOGAT) double-defective mutants could not.Glutamine synthetase (GS) and glutamate synthase (GOGAT) were formed constitutively in glutamate dehydrogenase (GDH)-defective mutants which grew and accumulated a small amount of glutamic acid in the glutamate fermentation medium containing high concentration of ammonia as the main nmitrogen source. When the cell-free extract of a glutamate dehydrogenase (GDH)-defective mutant was incubated with a mixture containing ammonium chloride, citrate and other substances for the glutamine synthetase (GS) and glutamate synthase (GOGAT) reactions, significant amount of glutamate was synthesized. These results suggested that glutamine synthetase (GS)/glutamate synthase (GOGAT) system could function for the assimilation of ammonia into glutamate in B. flavum.
- 社団法人日本生物工学会の論文
- 1985-02-25
著者
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Kumagai H
Kyoto Univ. Kyoto Jpn
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SUNG HA-CHIN
Department of Food Science and Techynology, Kyoto University
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TAKAHASHI MAKOTO
Department of Food Science and Technology, Kyoto University
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TAMAKI HISANORI
Department of Food Science and Technology, Kyoto University
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TACHIKI TAKASHI
Department of Food Science and Technology, Kyoto University
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KUMAGAI HIDEHIKO
Department of Food Science and Technology, Kyoto University
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TOCHIKURA TATSUROKURO
Department of Food Science and Technology, Kyoto University
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Kumagai Hidehiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Kumagai Hidehiko
Faculty Of Agriculture Kyoto University
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Tamaki H
Kyoto Univ. Kyoto Jpn
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Tamaki Hisanori
Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University
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Tamaki Hisanori
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Tochikura Tatsurokuro
Department Of Agricultural Chemistry Kyoto University
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Sung Ha-chin
School Of Agriculture Korea Univerasity
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Tachiki Takashi
Department Of Chemistry Ritsumeikan University
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Kumagai H
Department Of Agricultural Chemistry The University Of Tokyo
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Tachiki Takashi
Faculty Of Home Economics Kobe Women's University
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Tachiki Tkashi
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Tachiki Takashi
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Sung H‐c
Department Of Food Science And Technology Kyoto University
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Sung Ha-chin
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Takahashi Makoto
Department Of Coloproctological Surgery Juntendo University Faculty Of Medicine
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Takahashi M
Waseda Univ. Tokyo Jpn
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Sung H‐c
Korea Univ. Seoul Kor
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Tamaki Hisanori
Industrial Research Institute Of Ishikawa
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Takahashi Makoto
Department Of Applied Chemistry College Of Engineering Chubu University
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KUMAGAI Hidehiko
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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