Trehalose 6-Phosphate Production with Energy Coupling Fermentation by Yeast Cells

概要

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We tried a method for the procduction of trehalose 6-phosphate (T6P) with energy-coupling fermentation by baker's yeast. T6P was produced in a reaction mixture containing glucose, 5'-UMP, MgSO_4,inorganic phosphate, and dried cells of baker's yeast as the enzyme preparation. T6P was isolated from the reaction mixture and identified by TLC, HPLC, GC-MS, and enzymatic methods. The reaction conditions suitable for T6P production were investigated. The formation of T6P and its precursors, glucose 6-phosphate and UDP glucose, at various pHs and concentrations of substrates was examined. Accumulation of T6P was maximum with a reaction mixture containing 1 M glucose, 20 mM 5'-UMP, 20 mM MgSO_4,400 mM sodium phosphate buffer (pH 6.2), and 100 mg/ml dried cells of baker's yeast shaken at 37℃ for 6 h. The yield of T6P as a percentage of glucose was 11% (mol/mol) reaction under reaction conditions.
社団法人日本農芸化学会の論文
1998-04-23