Trehalase Activity and Trehalose Content in a Freeze-tolerant Yeast, Torulaspora delbrueckii, and Its Freeze-sensitive Mutant

スポンサーリンク

概要

• 論文の詳細を見る

• Two types of trehalase activities different in the optimum reaction pH (4.3 and 6.7) were found in a freeze-tolerant yeast, Torulaspora delbrueckii D2-4, and three types of the activities at pH 4.3, 5.7, and 6.7 were found in its freeze-sensitive mutant (60B3). The pH 4.3 activities in the cell extracts of the two strains were on the same level, but the pH 6.7 activity in the strain D2-4 was about half of that in the strain 60B3. The enzyme with the pH 4.3 activity and the other two enzymes were analogous to acid and neutral trehalases, respectively, in the sensitivity to metal ions and in response to phosphorylation and dephosphorylation. The trehalose content in the strain D2-4 was three times higher than that in the strain 60B3. The change of trehalose content during growth of the strain 60B3 correlated only to the change of the pH 5.7 activity.

• 社団法人日本農芸化学会の論文
• 1995-11-23

著者

• MURAKAMI Yoko

  Department of Food Science and Nutrition, Nara Women's University

• YOKOIGAWA Kumio

  Department of Food Science and Nutrition, Nara Women's University

• KAWAI Hiroyasu

  Department of Food Science and Nutrition, Nara Women's University

• Kumagai Hidehiko

  Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.

• Kumagai Hidehiko

  Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University

• Murakami Y

  Shizuoka Univ. Shizuoka Jpn

• Kawai Hiroyasu

  Department Of Food Science And Nutrition Nara Women's University

• Kumagai H

  Department Of Agricultural Chemistry The University Of Tokyo

• Yokoigawa K

  Nara Women's Univ. Nara Jpn

• Yokoigawa Kumio

  Department Of Food Science And Nutrition Nara Women's University

関連論文

• 2H14-5 グリコシル化した酵母 (Saccharomyces cerevisiae) のα-接合因子は高い接合活性を示す
• Crystallographic and Mutational Analyses of Substrate Recognition of Endo-α-N-acetylgalactosaminidase from Bifidobacterium longum
• Ammonia Assimilation by Glutamine Synthetase/Glutamate Syhthase System in Brevibacterium flavum
• Properties of Glutamate Synthase from Brevibacterium flavum
• Production and Preparation of Glutamate Synthase from Brevibacterium flavum
• Lipid Composition of Commercial Bakers' Yeasts Having Different Freeze-tolerance in Frozen Dough
• 664 Chemo-enzymatic synthesis of α-mating factor of Saccharomyces cerevisiae containing glutamine linked oligosaccharide and analyses of its activity
• Cloning of a Gene Encoding a Highly Stable Endo-β-1, 4-Glucanase from Aspergillus niger and Its Expression in Yeast
• Trypsin Inhibitory Activity of Bovine Fetuin De-O-glycosylated by Endo-α-N-acetylgalactosaminidase
• Transcriptional Regulation of Tyrosine Phenol-lyase Gene Mediated through TyrR and cAMP Receptor Protein
• Transglycosylation Activity of the Endo-β-1, 4-Glucanase from Aspergillus niger IFO31125 and Its Application
• Effects of Carbohydrate Chain on Surface Net Charge and Hydrophobicity of Glycoenzymes
• Structural Analysis of Disaccharides Synthesized by β-D-Glucosidase of Bifidobacterium breve clb and Their Assimilation by Bifidobacteria
• Transglycosylation Activity of β-N-Acetylhexosaminidase from Penixillium pxalicum and Its Application to Synthesis of a Drug Carrier
• Analysis of Low Temperature Inducible Mechanism of γ-Glutamyltranspeptidase of Escherichia coli K-12
• Cloning and Nucleotide Sequence of the β-D-Glucosidase Gene from Bifidobacterium breve clb, and Expression of β-D-Glucosidase Activity in Escherichia coli
• Mapping, Cloning, and DNA Sequencing of pepB Which Encodes Peptidase B of Escherichia coli K-12
• A Preliminary Description of the Crystal Structure of γ-Glutamyltranspeptidase from E. coli K-12
• Screening for Bacterial Strains Producing Lactate Oxidase
• Transcriptional Regulation of Tyrosine Phenol-Lyase Gene of Erwinia herbicola AJ2985
• Subunit Association of γ-Glutamyltranspeptidase of Escherichia coli K-12^1
• Formation of Lyso-glycosphingolipids by Streptomyces sp.
• Effect of Site-Directed Mutations on Processing and Activity of γ-Glutamyltranspeptidase of Escherichia coli K-12
• Efficient Production and Purification of Extracellular 1,2-α-L-Fucosidase of Bacillus sp. K40T
• Pulmonary infarction associated with bronchogenic carcinoma
• Deglycosylated Glucoamylase from Rhizopus niveus is Precipitated by Flavobacterium sp. Endo-β-N-acetylglucosaminidase(Microbiology & Fermentation Industry)
• Purification and Characterization of Thermostable α-Galactosidase from Ganoderma lucidum(Biochemistry & Molecular Biology)
• Purification and Properties of γ-Glutamyltranspeptidase from Penicillium roqueforti IFO 4622
• Purification and Properties of Glutaminase from Aspergillus oryzae
• Excretion and Rapid Purification of γ-Glutamyltranspeptidase from Escherichia coli K-12
• Unique Production of a Disaccharide, Galβ1→3GalNAc, by Endo-α-N-Acetylgalactosaminidase of Alcaligenes sp.
• Preservation of Raw Fish and Meat(Food & Nutrition)
• Peptide Production from Protein without Salt(Microbiology & Fermentation Industry)
• Detoxification Metabolism of o-Dinitrobenzene by the Yeast Issatchenkia orientalis(Microbiology & Fermentation Industry)
• Production and Localization of Enzymes on Soft Gel Cultivation(Microbiology & Fermentation Industry)
• Development of a Soft Gel Cultivation Method(Microbiology & Fermentation Industry)
• Induction and Efficient Purification of Endo-α-N-acetylgalactosaminidase from Alcaligenes sp.(Microbiology & Fermentation Industry)
• Synthesis of γ-Glutamyltaurine by γ-Glutamyltranspeptidase of Penicillium roqueforti(Microbiology & Fermentation Industry)
• S-Carboxymethylcysteine Synthase from Escherichia coli(Microbiology & Fermentation Industry)
• Formation of γ-Glutamyl Peptides by Glutaminase of Aspergillus oryzae(Microbiology & Fermentation Industry)
• Production of Deoxyribonucleoside Triphosphates through Coupled Fermentation with Energy Transfer(Microbiology & Fermentation Industry)
• Action of Glutaminase in a Model System of a Soy Sauce Fermentation(Microbiology & Fermentation Industry)
• Enzymatic Synthesis of γ-Glutamyltyrosine Methyl Ester from L-Glutamine and L-Tyrosine Methyl Ester with Escherichia coli K-12 γ-Glutamyltranspeptidase(Microbiology & Fermentation Industry)
• A Novel End-β-N-acetylglucosaminidase Acting on Complex Oligosaccharides of Glycoproteins in a Fungus (Microbiological Fermentation Industry)
• Solid-Phase Synthesis of N-Glycopeptide and Complex Glycopeptide Synthesis by Transglycosylation Reaction of Endoglycosidase
• Purification and Characterization of a Protease-Resistant Cellulase from Aspergillus niger
• Elucidation of the Role of Sugar Chains in Glycosylated-enzymes Using Endo-β-N-acetylglu-cosaminidase from Flavo-bacterium sp.
• Novel Specificities of Mucor hiemalis Endo-β-N-acetylglucosaminidase Acting Complex Asparagine-Linked Oligosaccharides
• Formation of γ-Glutamylglycylglycine by Extracellular Glutaminase of Aspergillus oryzae
• γ-Glutamyltranspeptidase Activity and the Properties of the Extracellular Glutaminase from Aspergillus oryzae(Microbiology & Fermentation Industry)
• Induction and Purification of α-L-Fucosidase from Fusarium oxysporum(Microbiology & Fermentation Industry)
• Distributionm, Formation and Stabilization of Yeast Glutathione S-transferase(Microbiology & Fermentation Industry)
• The Effect of Magnesium Ion on Yeast UDP-N-acetylglucosamine Pyrophosphorylase and Its Activation by Dithiothreitol
• Production of UDP-glucose by Torulopsis candida IFO 0768 : Studies on Microbial Metabolism of Sugar Nucleotides (VIII)
• Evaluation of an Alanine Racemase Gene as an Indicator for the Detection of Various Escherichia coli : Reactivity of the Gene Fragment with Various E. coli O157 : H7 Isolates
• Induction of Freeze-sensitive Mutants from a Freeze-tolerant Yeast, Torulaspora delbrueckii
• Mortality of Bifidobacteria in Boiled Yogurt
• Increase in β-D-Glucoside-assimilating Ability of Bifidobacterium breve 203 Due to Acclimation to β-D-Glucoside(Microbiology & Fermentation Industry)
• Hydrolysis of α-D-Galactosyl Oligosaccharides in Soymilk by α-D-Galactosidase of Bifidobacterium breve 203(Microbiology & Fermentation Industry)
• Isolation and Characterization of Two β-D-Glucosidases from Bifidobacterium breve 203(Microbiology & Fermentation Industry)
• p-Nitrophenyl Glycoside-hydrolyzing Activities in Bifidobacteria and Characterization of β-D-Galactosidase of Bifidobacterium longum 401(Microbiology & Fermentation Industry)
• Thermolabile Alanine Racemase from a Psychrotroph, Pseudomonas fluorescens : Purification and Properties
• Properties of an Extracellular Polysaccharide Produced by a Strain of Enterobacter Isolated from Pond Water
• Improving the Freeze Tolerance of Bakers' Yeast by Loading with Trehalose
• 各種銘柄干しシイタケの加熱過程における5'-ヌクレオチドの生成
• Distribution and Some Properties of Glutamine Synthetase and Glutamate Dehydrogenase in Bifidobacteria(Microbiology & Fermentation Industry)
• Isolation of Endo-α-N-acetylgalactos-aminidase from an Aerobic Bacterium(Microbiology & Fermentation Industry)
• Cloning of Alanine Racemase Genes from Pseudomonas fluorescens Strains and Oligomerization States of Gene Products Expressed in Escherichia coli(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• The Production of UDP-Galactose by Torulopsis candida IFO 0768 Grown on a Glucose Medium
• Isolation and Characterization of Freeze-tolerant Yeasts from Nature Available for the Frozen-dough Method(Microbiology & Fermentation Industry)
• Trehalase Activity and Trehalose Content in a Freeze-tolerant Yeast, Torulaspora delbrueckii, and Its Freeze-sensitive Mutant
• Suppressive Effect of Poly-γ-Glutamate on SOS Response of Salmonella typhimurium Induced by Chemical Mutagens(BREWING AND FOOD TECHNOLOGY)
• Simple Improvement in Freeze-Tolerance of Bakers' Yeast with Poly-γ-Glutamate(BREWING AND FOOD TECHNOLOGY)
• Phosphocholine-Containing Glycosyl Inositol-Phosphoceramides from Trichoderma viride Induce Defense Responses in Cultured Rice Cells
• 酒種パンに関する研究(1):酒種のパン生地醗酵力におよぼす培養条件の影響
• Suppressive Effects of the Extract from Sake Cake on Chemical Mutagen-Induced SOS Response in Salmonella typhimurium
• Hyperproduction of 3,4-Dihydroxyphenyl-L-alanine (L-Dopa) Using Erwinia herbicola Cells Carrying a Mutant Transcriptional Regulator TyrR
• A Rapid, Simple, and Effective Method of Constructing a Randomly Mutagenized Plasmid Library Free from Ligation
• トレハロースの負荷がパン酵母の冷凍耐性に及ぼす影響
• Difference between Escherichia coli O157:H7 and Non-Pathogenic E.coli : Survival and Growth in Seasonings
• A New Sterility Test for Cow's Milk Using Alanine Racemase Gene as the Index
• Saccharomyces属酵母の生育と発酵力に及ぼす塩化ナトリウムの影響
• Effect of Ethyl Alcohol on Growth and Intracellular Alanine Racemase of Psychrotrophs
• Trehalose Metabolism and Leavening Ability of Bakers' Yeast Grown in the Presence of Sodium Chloride
• Alamine Racemase Gene Fragments as Probes for Detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in Foods
• Trehalose 6-Phosphate Production with Energy Coupling Fermentation by Yeast Cells
• 塩化ナトリウムによる酒種の発酵力改善効果
• Structural Study of an Exocellular Polysaccharide of Bacillus circulans
• Production of a Novel Extracellular Polysaccharide by a Bacillus Strain Isolated from Soil
• Properties of a Highly Viscous Polysaccharide Produced by a Bacillus Strain Isolated from Soil
• Formation of Glutamine by Combination of Bifidobacterial Sugar Fermentation and Bacterial Glutamine Synthetase
• Purification and Characterization of β-D-Glucosidase (β-D-Fucosidase) from Bifidobacterium breve clb Acclimated to Cellobiose
• 114. 糖ヌクレオチドの醗酵生産とその代謝機構の関する研究
• パン酵母の冷凍障害と冷凍耐性酵母
• Kluyveromyces marxianus-based platform for direct ethanol fermentation and recovery from cellulosic materials under air-ventilated conditions(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Changes in Microbiota Population during Fermentation of Narezushi as Revealed by Pyrosequencing Analysis
• 136 酵母細胞壁多糖β(1→3)グルカン合成酵素の活性促進物質の単離同定
• Differences in the Substrate Specificities and Active-Site Structures of Two α-L-Fucosidases (Glycoside Hydrolase Family 29) from Bacteroides thetaiotaomicron
• Kluyveromyces marxianus-based platform for direct ethanol fermentation and recovery from cellulosic materials under air-ventilated conditions
• E1-1 Relation of allergic rhinitis and exercise-induced bronchoconstriction reffering to use of inhaled corticosteroids and leukotriene receptor antagonists : a cross-sectional study(English Session 1)

もっと見る 閉じる

スポンサーリンク