Effect of Ethyl Alcohol on Growth and Intracellular Alanine Racemase of Psychrotrophs
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概要
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The psychrotrophic alanine racemase from Pseudomonas fluorescens, a typical psychrotroph, is less resistant to organic solvents than the enzymes from thermophilic and mesophilic bacteria (Okubo et al., J.Home Econ.Jpn., 46 : 1135-1140, 1995). To further elucidate this difference, we examined the effect of ethyl alcohol on the growth and intracellular alanine racemase activity of three typical psychrotrophs-P.fluorescens, Bacillus psychrosaccharolyticus and B.psychrophilus-in comparison with two mesophiles, Escherichia coli and B.subtills. Although all the bacteria grew to the early stationary phase when cultivated at 22℃ for 36 h in the absence of ethyl alcohol, the growth of the psychrotrophs was more effectively suppressed by the addition of 3 and 5% ethyl alcohol to the medium than that of the mesophiles. The intracellular alanine racemase activity of the psychrotrophs was also more markedly reduced in the presence of ethyl alcohol than that of the mesophiles. When bacterial cells of each strain grown at 22℃ for 36 h in the absence of alcohol were suspended in 0-5% ethyl alcohol solution and incubated at 30℃ for 1 h, both the survival ratio and intracellular alanine racemase activity of the psychrotrophs were lower than those of the mesophiles. Thus, ethyl alcohol effectively reduced both the growth of the psychrotrophs and their intracellular alanine racemase activity. Low concentrations of various other alcohols also repressed the growth of the psychrotrophs at 10℃.
- 社団法人日本生物工学会の論文
- 1999-02-25
著者
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OKUBO Yoko
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Teikyo University
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Kumagai H
Kyoto Univ. Kyoto Jpn
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YOKOIGAWA Kumio
Department of Food Science and Nutrition, Nara Women's University
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KAWAI Hiroyasu
Department of Food Science and Nutrition, Nara Women's University
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Kumagai H
Nihon Univ. Fujisawa‐shi Jpn
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Kumagai Hidehiko
Faculty Of Agriculture Kyoto University
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Kumagai Hidehiko
Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.
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Kumagai Hidehiko
Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University
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河合 弘康
関西女子短期大学
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Kawai Hiroyasu
Department Of Food Science And Nutrition Nara Women's University
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Kumagai H
Department Of Agricultural Chemistry The University Of Tokyo
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Okubo Y
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Teikyo University
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Okubo Yoko
Department Of Ecological Engineering Toyohashi University Of Technology
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Kumagai Hidehiko
Graduate School Of Biostudies Kyoto University
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Yokoigawa K
Nara Women's Univ. Nara Jpn
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Yokoigawa Kumio
Department Of Food Science And Nutrition Nara Women's University
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Yokoigawa Kumiko
Department of Food Science and Nutrition, Nara Women's University
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