A Rapid, Simple, and Effective Method of Constructing a Randomly Mutagenized Plasmid Library Free from Ligation
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概要
- 論文の詳細を見る
The QuikChange site-directed mutagenesis methodology was applied to constructing a randomly mutagenized plasmid library simply by adding manganese to the reaction mixture. This method is superior to the normally employed Pol I-type polymerase-based error-prone PCR in that (i) it does not require a subsequent ligation reaction, and (ii) there is no accumulation of mutations at the same site. α-Complementation analysis and subsequent sequence analyses of the lacZα genes in the mutated library revealed that the mutations occurred randomly within the target gene and involved all possible base substitutions.
- 社団法人 日本農芸化学会の論文
- 2008-04-23
著者
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Katayama Takane
Research Institute for Bioresource and Biotechnology, Ishikawa Prefectural University
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KUMAGAI Hidehiko
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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Katayama Takane
Graduate School Of Biostudies Kyoto University
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Kumagai H
Kyoto Univ. Kyoto Jpn
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Kumagai Hidehiko
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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Tanaka K
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Tanaka K
Tottori Univ. Tottori
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Kumagai H
Nihon Univ. Fujisawa‐shi Jpn
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Kumagai Hidehiko
Faculty Of Agriculture Kyoto University
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Koyanagi Takashi
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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Kumagai Hidehiko
Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.
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Kumagai Hidehiko
Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University
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Tanaka Kiyoshi
Coll. Environ. Health Azabu Univ.
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河合 弘康
関西女子短期大学
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Kumagai H
Department Of Agricultural Chemistry The University Of Tokyo
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Kumagai Hidehiko
Graduate School Of Biostudies Kyoto University
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YOSHIDA Erina
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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MINAMI Hiromichi
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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Yoshida Erina
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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Kikuchi Takao
Coll. Environ. Health Azabu Univ.
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Minami Hiromichi
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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Koyanagi Takashi
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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Tanaka Kyoko
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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