Hyperproduction of 3,4-Dihydroxyphenyl-L-alanine (L-Dopa) Using Erwinia herbicola Cells Carrying a Mutant Transcriptional Regulator TyrR
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概要
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In the last few decades, enzymatic production of 3,4-dihydroxyphenyl-L-alanine (L-dopa) using tyrosine phenol-lyase (Tpl) has been industrialized. This method has an intrinsic problem of tyrosine contamination because Tpl is synthesized under tyrosine-induced conditions. Herein, we constructed a hyper-L-dopa-producing strain by exploiting a mutant TyrR, an activator of tpl. The highest productivity was obtained for the strain grown under non-induced conditions. It was 30-fold higher than that obtained for tyrosine-induced wild-type cells.
著者
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Yokozeki Kenzo
AminoScience Laboratories, Ajinomoto Co., Inc.
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Katayama Takane
Research Institute for Bioresource and Biotechnology, Ishikawa Prefectural University
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KUMAGAI Hidehiko
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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Katayama Takane
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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Koyanagi Takashi
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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Suzuki Hideyuki
Graduate School of Biostudies, Kyoto University
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ONISHI Akiko
Graduate School of Biostudies, Kyoto University
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