Cloning of the Gene Encoding α-Methylserine Hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and Characterization of the Recombinant Enzyme
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概要
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Genes encoding α-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 were cloned and expressed in Escherichia coli. The purified enzymes were homodimers with a 46-kDa subunit and contained 1 mol/mol-subunit of pyridoxal 5′-phosphate. The Vmax of these enzymes catalyzing the conversion of α-methyl-L-serine to D-alanine via tetrahydrofolate was 22.1 U/mg (AJ110403) and 15.4 U/mg (AJ110404).
- 社団法人 日本農芸化学会の論文
著者
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Yokozeki Kenzo
AminoScience Laboratories, Ajinomoto Co., Inc.
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Nozaki Hiroyuki
Aminoscience Laboratories Ajinomoto Co. Ltd.
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KURODA Shinji
AminoScience Laboratories, Ajinomoto Co., Inc.
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WATANABE Kunihiko
AminoScience Laboratories, Ajinomoto Co., Inc.
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