Cloning of the Xylitol Dehydrogenase Gene from Gluconobacter oxydans and Improved Production of Xylitol from D-Arabitol(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Xylitol dehydrogenase (XDH) was purified from the cytoplasmic fraction of Gluconobacter oxydans ATCC 621. The purified enzyme reduced D-xylulose to xylitol in the presence of NADH with an optimum pH of around 5.0. Based on the determined NH_2-terminal amino acid sequence, the gene encoding xdh was cloned, and its identity was confirmed by expression in Escherichia coli. The xdh gene encodes a polypeptide composed of 262 amino acid residues, with an estimated molecular mass of 27.8 kDa. The deduced amino acid sequence suggested that the enzyme belongs to the short-chain dehydrogenase/reductase family. Expression plasmids for the xdh gene were constructed and used to produce recombinant strains of G. oxydans that had up to 11-fold greater XDH activity than the wildtype strain. When used in the production of xylitol from D-arabitol under controlled aeration and pH conditions, the strain harboring the xdh expression plasmids produced 57 g/l xylitol from 225 g/l D-arabitol, whereas the control strain produced 27 g/l xylitol. These results demonstrated that increasing XDH activity in G. oxydans improved xylitol productivity.
- 社団法人日本農芸化学会の論文
- 2003-03-23
著者
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Yokozeki Kenzo
AminoScience Laboratories, Ajinomoto Co., Inc.
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Suzuki S
Department Of Chemistry Graduate School Of Science Osaka University
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Suzuki S
Aminoscience Laboratories Ajinomoto Co. Inc.
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Tonouchi N
Ajinomoto. Co. Inc. Kawasaki Jpn
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Yokozeki K
Ajinomoto. Co. Inc. Kawasaki Jpn
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Yokozeki Kenzo
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Yokozeki Kenzo
Aminoscience Laboratories Ajinomoto Co. Inc.
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TONOUCHI Naoto
AminoScience Laboratories, Ajinomoto Co., Inc.
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SUGIYAMA Masakazu
AminoScience Laboratories, Ajinomoto Co., Inc.
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Tonouchi Naoto
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Tonouchi Naoto
Bio-polymer Research Co. Ltd. Ksp R&d Business-park Bldg.
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Suzuki Shunichi
Central Research Laboratories Ajinomoto Co. Inc.
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SUZUKI Shun-ichi
Aminoscience Laboratories, Ajinomoto Co., Ltd.
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Suzuki Shun-ichi
Aminoscience Laboratories Ajinomoto Co. Ltd.
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Sugiyama Minoru
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Sugiyama M
Aminoscience Laboratories Ajinomoto. Co. Inc.
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