Screening of Microorganisms Producing D-p-Hydroxyphenyl-glycine from DL-5-(p-Hydroxyphenyl)hydantoin(Microbiology & Fermentation Industry)

概要

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Microorganisms that symmetrically produce D-p-hydroxyphenylglycine (D-HPG) from DL-5-(p-hydroxyphenyl)hydantoin (DL-HPH), an intermediate in the chemical synthesis of DL-HPG, were screened from stock cultures and soil samples. Of the 430 strains of bacteria, yeasts and actionomycetes obtained from our stock cultures, only two, Achromobacter delicatulus AJ-2446 and Achromobacter dendriticum AJ-2447, were capable of producing D-HPG from DL-HPH, but the yields were rather low. A bacterium with high productivity was isolated from a soil sample. It was classified as a bacterium belonging to the genus, Pseudomonas. In the presence of intact cells of Pseudomonas sp.Aj-11220 isolated from soil, which was selected as the best strain, D-HPG was produced from D-, L- and DL-HPH at a molar yield of more than 90%. This suggests that racemization and asymmetric hydrolysis occurred simultaneously in this reaction system.
社団法人日本農芸化学会の論文
1987-02-23