Enzymatic Production of L-Tryptophan from DL-5-Indolylmethylhydantoin by Mutants of Flavobacterium sp. T-523(Microbiology & Fermentation Industry)

概要

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A bacterium isolated from soil, T-523, which hydrolyzes DL-5-indolylmethylhydantoin to L-tryptophan, was classified as a bacterium belonging to the genus, Flavobacterium. The prevention of tryptophan degradation by Flavobacterium sp.AJ-3912 (T-523) was investigated for improvement of the L-tryptophan production, because the L-tryptophan accumulated on enzymatic production from DL-5-indolylmethylhydantoin was partially degraded. L-Tryptophan production by Flavobacterium Sp.AJ-3912 increased with the addition of agents that inhibit tryptophan oxygenase, such as hydroxylamine and phenylhydrazin, to the reaction mixture. In the presence of an appropriate concentration of hydroxylamine, the amount of L-tryptophan produced from L-5-indolylmethylhydantoin reached 8.8 mg/ml, with a molar yield of 99% (in the absence of hydroxylamine, 7.1 mg/ml with a molar yield of 80%). The L-tryptophan productivity was also improved with the use of mutants with a genetically blocked tryptophan degradation pathway. In the presence of intact cells of a representative mutant, AJ-3940, L-tryptophan was produced with a molar yield of 97%.
社団法人日本農芸化学会の論文
1987-02-23