Novel Enzymatic Method for the Production of Xylitol from D-Arabitol by Gluconobacter oxydans(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Microorganisms capable of producing xylitol from D-arabitol were screened for. Of the 420 strains tested, three bacteria, belonging to the genera Acetobacter and Gluconobacter, produced xylitol from D-arabitol when intact cells were used as the enzyme source. Among them, Gluconobacter oxydans ATCC 621 produced 29.2 g/l xylitol from 52.4 g/l D-arabitol after incubation for 27 h. The production of xylitol was increased by the addition of 5% (v/v) ethanol and 5 g/l D-glucose to the reaction mixture. Under these conditions, 51.4 g/l xylitol was obtained from 52.4 g/l D-arabitol, a yield of 98%, after incubation for 27 h. This conversion consisted of two successive reactions, conversion of D-arabitol to D-xylulose by a membrane-bound D-arabitol dehydrogenase, and conversion of D-xylulose to xylitol by a soluble NAD-dependent xylitol dehydrogenase. Use of disruptants of the membrane-bound alcohol dehydrogenase genes suggested that NADH was generated via NAD-dependent soluble alcohol dehydrogenase.
- 社団法人日本農芸化学会の論文
- 2002-12-23
著者
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Yokozeki Kenzo
AminoScience Laboratories, Ajinomoto Co., Inc.
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Suzuki S
Department Of Chemistry Graduate School Of Science Osaka University
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Suzuki S
Aminoscience Laboratories Ajinomoto Co. Inc.
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Yokozeki K
Ajinomoto. Co. Inc. Kawasaki Jpn
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Yokozeki Kenzo
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Yokozeki Kenzo
Aminoscience Laboratories Ajinomoto Co. Inc.
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SUGIYAMA Masakazu
AminoScience Laboratories, Ajinomoto Co., Inc.
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Suzuki Shunichi
Central Research Laboratories Ajinomoto Co. Inc.
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Hashiguchi Ken-ichi
Aminoscience Laboratories Ajinomoto Co. Inc.
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SUZUKI Shun-ichi
Aminoscience Laboratories, Ajinomoto Co., Ltd.
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Mihara Yasuhiro
Aminoscience Laboratories Ajinomoto Co. Inc.
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Suzuki Shun-ichi
Aminoscience Laboratories Ajinomoto Co. Ltd.
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Sugiyama Minoru
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Sugiyama M
Aminoscience Laboratories Ajinomoto. Co. Inc.
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